Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-25T18:32:42.115Z Has data issue: false hasContentIssue false

The expression of the gene asebia in the laboratory mouse: I. Epidermis and dermis

Published online by Cambridge University Press:  14 April 2009

Wendy J. Josefowicz
Affiliation:
Department of Biomedical Sciences, University of Guelph, Guelph, CanadaN1G 2W2
Margaret H. Hardy
Affiliation:
Department of Biomedical Sciences, University of Guelph, Guelph, CanadaN1G 2W2
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Mice homozygous for the asebia mutation (ab/ab), which have defective sebaceous glands, display abnormalities in several other aspects of the integument. Histological sections showed that hyperplasia of the cellular layers and the stratum corneum of the epidermis is apparent at birth and increases markedly with age. Enlarged intercellular spaces are also noted in the epidermis. The thicker dermal layer of the asebic mice is characterized by increased vascularity, increased cellularity and the abnormal morphology of a large proportion of the ‘fibroblast’ population. Electron microscopy demonstrated the many abnormalities in the dermal fibroblasts as well as large amounts of cellular debris in the surrounding matrix. Collagen and elastin show alterations at the light microscopic and ultrastructural levels. Many features of the asebic dermis resemble those found with mild inflammation and with the lysosomal storage diseases. Changes in the dermis of asebic foetuses were noted prior to epidermal alterations and may mediate the latter.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCES

Billingham, R. E. & Silvers, W. K. (1967). Studies on the conservation of epidermal specificities of skin and certain mucosas in adult mammals. Journal of Experimental Medicine 125, 429446.CrossRefGoogle ScholarPubMed
Brady, R. O. & King, F. M. (1973). Gaucher's disease. In Lysosomes and Storage Diseases (ed. Hers, H. G. and Van Hoof, F.), pp. 381394. New York and London: Academic Press.Google Scholar
Carter, H. B. & Clarke, W. H. (1957). The hair follicle group and skin follicle population of Australian Merino sheep. Australian Journal of Agricultural Research 8, 91108.CrossRefGoogle Scholar
Chase, H. B., Montagna, W. & Malone, J. D. (1953). Changes in the skin in relation to the hair growth cycle. Anatomical Record 116, 7581.CrossRefGoogle Scholar
Decker, J. & Sercarz, E. (1975). Antigen binding cells and the generation of diversity. American Zoologist 15, 189197.CrossRefGoogle Scholar
Dry, F. W. (1926). The coat of the mouse (Mus musculus). Journal of Genetics 16, 287340.CrossRefGoogle Scholar
Gates, A. H., Arundell, F. D. & Karasek, M. A. (1969). Hereditary defect of the pilosebaceous unit in a new double mutant mouse. Journal of Investigative Dermatology 52, 115118.CrossRefGoogle Scholar
Gates, A. H. & Karasek, M. A. (1965). Hereditary absence of sebaceous glands in the mouse. Science, N.Y. 148, 14711473.CrossRefGoogle ScholarPubMed
Grüneberg, H. (1943). The development of some external features in mouse embryos. Journal of Heredity 34, 8892.CrossRefGoogle Scholar
Hers, H. G. (1973). The concept of inborn lysosomal disease. In Lysosomes and Storage Diseases (ed. Hers, H. G. and Van Hoof, F.), pp. 147171. New York and London: Academic Press.Google Scholar
Jackson, D. S. (1968). Biological function of collagen in the dermis. In Advances in Biology of Skin. Vol. X. The Dermis (ed. Montagna, W., Bentley, J. P. and Dobson, R. L.), pp. 3948. New York: Appleton-Century-Crofts.Google Scholar
Josefowicz, W. J. (1975). The Development and Expression of the Asebia Mutation in Mice. M.Sc. Thesis, University of Guelph, Guelph, Canada.Google Scholar
Josefowicz, W. J. & Hardy, M. (1974). The development and expression of the mutation asebia ab/ab in mice. Proceedings of the Canadian Federation of Biological Societies 17, 173.Google Scholar
Josefowicz, W. J. & Hardy, M. H. (1978 a). The expression of the gene asebia in the laboratory mouse. II Hair follicles. Genetical Research (in press).CrossRefGoogle Scholar
Josefowicz, W. J. & Hardy, M. H. (1978 b). The expression of the gene asebia in the laboratory mouse. III Sebaceous glands. Geneticcl Research (in press).CrossRefGoogle Scholar
Lloyd, J. B. (1973). Experimental support for the concept of lysosomal storage disease. In Lysosomes and Storage Diseases (ed. Hers, H. G. and Van Hoof, F.), 173195. New York and London: Academic Press.Google Scholar
Luft, J. H. (1961). Improvement in epoxy resin embedding techniques. Journal of Biophysical and Biochemical Cytology 9, 409414.CrossRefGoogle Scholar
McManus, J. F. A. & Mowry, R. W. (1965). Staining Methods, Histological and Histochemical. New York, Evanston and London: Harper and Row.Google Scholar
Muir, H. (1973). Structure and enzymic degradation of mucopolysaccharides. In Lysosomes and Storage Diseases (ed. Hers, H. G. and Van Hoof, F.), pp. 79104. New York and London: Academic Press.Google Scholar
Pinkus, H. (1944). Acid Orcein-Giemsa stain (modification of Unna-Taenzer method); useful routine stain for dermatologic sections. Archives of Dermatology and Syphilology 49, 355356.CrossRefGoogle Scholar
Reynolds, E. S. (1963). The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology 17, 208225.CrossRefGoogle ScholarPubMed
Rugh, R. (1968). The Mouse, Its Reproduction and Development. Minneapolis: Burgess Publishing Co.Google Scholar
Stern, J., Novikoff, A. B. & Terry, R. D. (1972). In Sphingolipids, Sphingolipidoses and Allied Disorders (ed. Volk, B. and Aronson, S. M.), p. 651. New York: Plenum.CrossRefGoogle Scholar
Suzuki, K. & Suzuki, K. (1973). Krabbe's disease. In Lysosomes and Storage Diseases (ed. Hers, H. G. and Van Hoof, F.), pp. 395410. New York and London: Academic Press.Google Scholar
Voorhees, J. J., Marcelo, C. L. & Duell, E. A. (1975). Cyclic AMP, cyclic GMP, and glucocorticoids as potential metabolic regulators of epidermal proliferation and differentiation. Journal of Investigative Dermatology 65, 179190.CrossRefGoogle ScholarPubMed
Wells, P. W. & Eyre, P. (1972). The pharmacology of passive cutaneous anaphylaxis in the calf. Canadian Journal of Physiology and Pharmacology 50, 255262.CrossRefGoogle ScholarPubMed
Wessells, N. K. (1967). Differentiation of epidermis and epidermal derivatives. New England Journal of Medicine 277, 2133.CrossRefGoogle ScholarPubMed
Willoughby, D. A. & Di Rosa, M. (1971). A unifying concept for inflammation: a new appraisal of some old mediators. In Immunopathology of Inflammation (ed. Forscher, B. K. and Houck, J. C.), pp. 2838. Amsterdam: Excerpta Medica.Google Scholar