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Expression and amplification of the genes for ribosomal RNA in bobbed mutants of Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

F. Lee Dutton  and
Hallie M. Krider
F. Lee Dutton
Affiliation:
Biological Sciences Group, Molecular Genetics and Cell Biology Section, The University of Connecticut, Storrs, CT 06268
Hallie M. Krider
Affiliation:
Biological Sciences Group, Molecular Genetics and Cell Biology Section, The University of Connecticut, Storrs, CT 06268
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We have employed stocks bearing clonally derived X chromosomes to investigate several features of the bobbed mutant syndrome, and the amplification of rDNA genes in D. melanogaster. We report that posterior macroscutellar bristle length correlates well with the rDNA content (i.e. dose of ivs–, or uninterrupted genes) in cloned X derivative strains. X/O males and X/X females with statistically indistinguishable rDNA contents have virtually identical bristle lengths. This indicates that (with respect to this phenotypic character) the rDNAs in these two genotypes are expressed equally, without apparent sexual dimorphism or dosage compensation. However, the severity of bobbed phenotype in terms of bristle morphology, turgite etching, and delayed eclosion is greater in the Xbb/XNO− female than in the Xbb/O male genotype for the alleles examined. We estimate the minimum dose of functioning rRNA genes required for viability at 26 δC to be 70 genes per diploid genome. We have examined the capacity of several X chromosomes which bear bobbed mutant alleles to compensate in X/O males, and find that disproportionate replication of these rDNAs does not take place. In contrast, at least one of the non-compensating bobbed alleles does appear to undergo rDNA magnification.

Type
Research Article
Information
Genetics Research , Volume 45 , Issue 2 , April 1985 , pp. 155 - 165
Copyright
Copyright © Cambridge University Press 1985

References

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