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Evidence for polarity of chromosome replication in F− strains of Escherichia coli

Published online by Cambridge University Press:  14 April 2009

W. D. Donachie  and
Millicent Masters
W. D. Donachie
Affiliation:
Medical Research Council, Microbial Genetics Research Unit, Hammersmith Hospital, Ducane Road, London, W.12
Millicent Masters
Affiliation:
Medical Research Council, Microbial Genetics Research Unit, Hammersmith Hospital, Ducane Road, London, W.12
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The inducibility of three enzymes (β-galactosidase, tryptophanase and D-serine deaminase) has been measured at various times during the cell cycles of three strains of Escherichia coli (K12 58–161 F−, B/r F– and 15T−). In each strain sharp increases in inducibility of these enzymes occurred at characteristic periods in each cell cycle. Such increases depend on DNA replication and therefore probably reflect synchronized gene replication. It is inferred that chromosome replication in these F− strains is sequential from a fixed origin.

Infection with F′Lack+ results in an extra period of increase in inducibiity of β-galactosidase in each cell cycle. It is concluded that the F′ episome replicates once in each cell cycle at a time soon after cell separation.

Type
Short Papers
Information
Genetics Research , Volume 8 , Issue 1 , August 1966 , pp. 119 - 124
Copyright
Copyright © Cambridge University Press 1966

References

REFERENCES

Abbo, F. E. & Pardee, A. B. (1960). Synthesis of macromolecules in synchronously dividing bacteria. Biochim. biophys. Acta, 39, 478–485.CrossRefGoogle Scholar
Cairns, J. (1963). The bacterial chromosome and its manner of replication as seen by auto-radiography. J. molec. Biol. 6, 208–213.CrossRefGoogle Scholar
Donachie, W. D. (1964). The regulation of pyrimidine biosynthesis in Neurospora crassa. II. Heterokaryons and the role of the ‘regulatory mechanisms’. Biochim. biophys. Acta, 82, 293–302.CrossRefGoogle Scholar
Garen, A. & Garen, S. (1963). Complementation in vivo between structural mutants of alkaline phosphatase from E. coli. J. molec. Biol. 7, 13–22.CrossRefGoogle ScholarPubMed
Jacob, F., Schaeffer, P. & Wollman, E. L. (1960). Episomic elements in bacteria. Soc. Gen. Microbiol. 10th Symp., pp. 67–89. London: Cambridge Univ. Press.Google Scholar
Kuempel, P., Masters, M. & Pardee, A. B. (1965). Bursts of enzyme synthesis in the bacterial duplication cycle. Biochem. biophys. Res. Commun. 18, 858–867.CrossRefGoogle Scholar
Lark, K. G. & Bird, R. E. (1965). Segregation of the conserved units of DNA in Escherichia coli. Proc. natn. Acad. Sci. U.S.A. 54, 1444–1450.CrossRefGoogle ScholarPubMed
Masters, M., Kuempel, P. & Pardee, A. B. (1964). Enzyme synthesis in synchronous cultures of bacteria. Biochem. biophys. Res. Commun. 15, 38–42.CrossRefGoogle ScholarPubMed
Mitchison, J. M. & Vincent, W. S. (1965). Preparation of synchronous cell cultures by sedimentation. Nature, Lond. 205, 987–989.CrossRefGoogle Scholar
Nagata, T. (1963). The molecular synchrony and sequential replication of DNA in Escherichia coli. Proc. natn. Acad. Sci. U.S.A. 49, 551–559.CrossRefGoogle ScholarPubMed
Pardee, A. B. & Prestidge, L. S. (1955). Induced formation of serine and threonine deaminases by Escherichia coli. J. Bact. 70, 667–674.CrossRefGoogle ScholarPubMed
Pardee, A. B. & Prestidge, L. S. (1961). The initial kinetics of enzyme induction. Biochim. biophys. Acta, 49, 77–88.CrossRefGoogle ScholarPubMed
Pittard, J. & Ramakrishnan, T. (1964). Gene transfer by F′ strains of Escherichia coli. IV. Effect of chromosomal deletion on chromosome transfer. J. Bad. 88, 367–373.Google ScholarPubMed
Rownd, R. (1965). Reported in discussion at the 15th Symposium of the Society for General Microbiology, London.Google Scholar
Taylor, A. L. & Thoman, M. S. (1964). The genetic map of Escherichia coli K12. Genetics, 50, 659–677.CrossRefGoogle Scholar
Yoshikawa, H. & Sueoka, N. (1963). Sequential replication of the Bacillus subtilis chromosome. Proc. natn. Acad. Sci. U.S.A. 49, 559–566.CrossRefGoogle ScholarPubMed