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Enrichment and screening of heat-sensitive mutants of Physarum polycephalum

Published online by Cambridge University Press:  14 April 2009

Peter Sudbery ,
Kari Haugli  and
Finn Haugli
Peter Sudbery
Affiliation:
Institute of Medical Biology, University of Tromsø, 9000 Tromsø, Norway
Kari Haugli
Affiliation:
Institute of Medical Biology, University of Tromsø, 9000 Tromsø, Norway
Finn Haugli
Affiliation:
Institute of Medical Biology, University of Tromsø, 9000 Tromsø, Norway
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Summary

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A new method for the isolation of temperature-sensitive mutants of Physarum polycephalum is described. It involves enrichment and prescreening of mutagenized amoebae followed by screening at both the plasmodial and amoebal stage. A total of 74 temperature-sensitive strains were recovered of which 26 were temperature-sensitive only as plasmodia, 35 only as amoebae and 13 in both stages. After a shift to the nonpermissive temperature, DNA and protein synthesis were followed in temperature-sensitive plasmodia to discover if the lesion affected functions of the nuclear cycle.

Type
Research Article
Information
Genetics Research , Volume 31 , Issue 1 , February 1978 , pp. 1 - 12
Copyright
Copyright © Cambridge University Press 1978

References

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