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Effects of the white allele of the mi locus on coat pigmentation in chimeric mice

Published online by Cambridge University Press:  14 April 2009

Boris V. Konyukhov ,
Boris N. Kindyakov  and
Natalia A. Malinina
Boris V. Konyukhov
Affiliation:
Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina 3, 117809, Moscow, Russia
Boris N. Kindyakov
Affiliation:
Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklay 16/10, 117871, Moscow, Russia
Natalia A. Malinina
Affiliation:
Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina 3, 117809, Moscow, Russia
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To investigate the cellular action of the Miωh allele in the mouse with regard to its effects upon coat color patterns, we generated a series of aggregation chimeras, using embryos that differ in their mi locus genotype. We have obtained 11 chimeras Miωh/ + C/C↔ + / + c/c and 8 chimeras + / + C/C↔ + / + c/c. Chimerism was determined by coat and retinal pigment epithelium mosaicism and by the electrophoretic analysis of GPI-1 isoenzymes. In Miωh/+ C/C↔, +/+ c/c mice white coat color prevailed due to the higher percentage of unpigmented areas and the higher percentage of unpigmented hairs in pigmented areas. Our data indicate that a single Miωh gene dose decreases the melanoblast proliferative activity, causing the lightening of coat pigmentation. In Miωh/ + C/C↔+/+ c/c mice a few pigmented hairs were often detected on the belly where Miωh / + mice always had a white spot. This suggests that in the chimeras the presence of some non-Miωh cells in the skin of the belly allows pigment cells to develop. Using embryos of two substrains of Miωh/Miωh mice that differ in their Gpi-1 locus genotype we have produced 8 Miωh/ + ↔ Miωh/Miωh chimeras. In all these chimeras coat color patterns resembled those of Miωh/ + heterozygotes despite the higher percentage of the Miwh/Miωh component in three chimeras. Mosaic hairs were absent in the chimeras. This shows that the chimeras have only one Miωh/ + melanoblast population which actively proliferates and colonizes almost all hair follicles. Thus the Miωh/Miωh dermis and epidermis do not suppress proliferation and differentiation of the Miωh/ + melanoblasts except the certain area on the belly.

Type
Research Article
Information
Genetics Research , Volume 63 , Issue 3 , June 1994 , pp. 175 - 181
Copyright
Copyright © Cambridge University Press 1994

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