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The effects of dark holding and photoreactivation on ultraviolet light-induced mitotic recombination and survival in yeast

Published online by Cambridge University Press:  14 April 2009

James M. Parry  and
B. S. Cox
James M. Parry
Affiliation:
Botany School, South Parks Road, Oxford
B. S. Cox
Affiliation:
Botany School, South Parks Road, Oxford
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UV induces lesions in DNA which lead to the death of cells, mutation and, in yeast, intragenic and intergenic mitotic recombination. We have investigated the interaction of two post-treatments, dark holding and photoreactivation, on the frequencies of these events. It was found that dark holding reduces cell death and intergenic recombinants, but causes an increase in intragenic recombination frequency. Photoreactivation reduces cell death and intragenic recombination, but has no effect on intergenic recombination. After dark holding, photoreactivation has no further effect on cell survival or intergenic recombination, but may reduce the frequency of intragenic recombinants. After photoreactivation, dark holding still causes an increase in cell survival and the frequency of intragenic recombination, and reduces the frequency of intergenic recombination.

Type
Research Article
Information
Genetics Research , Volume 12 , Issue 2 , October 1968 , pp. 187 - 198
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Boyce, R. P. & Howard-Flanders, P. (1964). Release of ultraviolet light-induced thymine dimers from DNA in Escherichia coli K-12. Proc. natl. Acad. Sci., U.S.A. 51, 293–300.CrossRefGoogle Scholar
Cox, B. S. & Bevan, E. A. (1962). Aneuploidy in yeast. New Phytol. 61, 342–355.CrossRefGoogle Scholar
Hatchard, C. G. & Parker, C. A. (1956). A new sensitive chemical actinometer. II. Potassium ferrioxalate as a standard chemical actinometer. Proc. Roy. Soc. A 235, 518–536.Google Scholar
Holliday, R. (1962). Effect of photoreactivation on ultraviolet-induced segregation of a heterozygous diploid. Nature, Land. 193, 95–96.CrossRefGoogle Scholar
Holliday, R. (1964). A mechanism for gene conversion in fungi. Genet. Res. Camb. 5, 282–304.CrossRefGoogle Scholar
Howard-Flanders, P. & Boyce, R. P. (1966). DNA repair and genetic recombination: studies of mutants of Escherichia coli defective in these processes. Radiation Res. Suppl. 6, 156–184.Google Scholar
Mortimer, R. K. & Hawthorne, D. C. (1963). Genetic mapping in Saccharomyces. Genetics 63, 165–173.Google Scholar
Parry, J. M. (1966). Ph.D. Thesis, Liverpool.Google Scholar
Parry, J. M. & Cox, B. S. (1965). Photoreactivation of UV induced reciprocal recombination, gene conversion and mutation to prototrophy in Saccharomyces cerevisiae. J. gen. Microbiol. 40, 235–241.CrossRefGoogle Scholar
Parry, J. M. & Cox, B. S. (1968). Mitotic recombination induced by ultraviolet light in synchronous cultures of yeast. Submitted.CrossRefGoogle Scholar
Patrick, M. H., Haynes, R. H. & Uretz, R. B. (1964). Dark recovery phenomena in yeast. I. Comparative effects with various inactivating agents. Radiation Res. 21, 144–163.CrossRefGoogle ScholarPubMed
Petijohn, D. & Hanawalt, P. C. (1964). Evidence for repair replication of UV damaged DNA in bacteria. J. molec. Biol. 9, 395–410.CrossRefGoogle Scholar
Roman, H. (1956). Studies of gene mutation in Saccharomyces. Cold Spring Harb. Symp. quant. Biol. 21, 175–183.CrossRefGoogle ScholarPubMed
Roman, H. & Jacob, F. (1958). A comparison of spontaneous and ultraviolet induced alleilc recombination with reference to the recombination of outside markers. Cold Spring. Harb. Symp. quant. Biol. 23, 155–160.CrossRefGoogle Scholar
Rupert, C. S. (1964). Photoreactivation of ultraviolet damage. In Photophysiology, 2, 283–287. ed. Giese, A. C.. New York: Academic Press.CrossRefGoogle Scholar
Setlow, J. K. (1966). The molecular basis of biological effects of UV radiation and photo-reactivation. In Current topics in Radiation Research, ed. Ebert, M. and Howard, A., 2, 196. Amsterdam: North-Holland Publ. Co.Google Scholar
Setlow, J. K., Boling, M. E. & Bollom, F. J. (1965). The chemical nature of photoreactivable lesions in DNA. Proc. natl. Acad. Sci., U.S.A. 53, 1430–1436.CrossRefGoogle ScholarPubMed
Setlow, R. B. & Carrier, W. L. (1964). The disappearance of thymine dimers from DNA: an error correcting mechanism. Proc. natl. Acad. Sci., U.S.A. 51, 226–231.CrossRefGoogle ScholarPubMed
Whitehouse, H. L. K. & Hastings, P. J. (1965). The analysis of genetic recombination on the polaron hybrid DNA model. Genet. Res. Camb. 6, 27–92.CrossRefGoogle ScholarPubMed
Witkin, E. (1966). Radiation-induced mutations and their repair. Science 152, 1345–1353.CrossRefGoogle ScholarPubMed