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Effects of a temperature-sensitive Minute mutation on gene expression in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Donald A. R. Sinclair ,
Thomas A. Grigliatti  and
Thomas C. Kaufman
Donald A. R. Sinclair
Affiliation:
Department of Zoology, University of British Columbia, Vancouver, B.C. V6T 2A9Canada.
Thomas A. Grigliatti
Affiliation:
Department of Zoology, University of British Columbia, Vancouver, B.C. V6T 2A9Canada.
Thomas C. Kaufman
Affiliation:
Department of Biology, Indiana University, Bloomington, Indiana, U.S.A.

Summary

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Minute (M) lesions exhibit a striking propensity for interacting with many different mutations. In the past, few attempts have been made to explain these diverse phenomena. This study describes a variety of temperature-sensitive (ts) interactions exhibited by the ts third chromosome Minute mutation M(3)LS4Q-III (Q-III). Most of these interactions (i.e. those involving vg, cp, Dl, Dfd or Ly) reflect Q-III-induced enhancement of the respective mutant phenotypes at the restrictive temperature. However, Q-III also suppresses the extra-sex-comb phenotypes of Pc and Msc at 29 °C and evokes lethal and bristle traits when combined with J34e at the restrictive temperature. All of these interactions are characteristic of non-ts Minute lesions and thus they appear to be correlated with general physiological perturbations associated with the M syndrome. In addition, our findings show that mutations that affect ribosome production and/or function, namely su(f)ts67g and bbts−1, exhibit interactions comparable to those elicited by Q-III. Hence, in accordance with previous findings, we argue that most of the Q-III interactions can be attributed to reduced translational capacity at the restrictive temperature. Finally, reciprocal temperature shift studies were used to delineate TSPs for interactions between Q-III and vg (mid to late second instar), cp (about mid-third instar), Dfd (early third instar) and Dl (late second to mid third instar). We believe that these TSPs represent developmental intervals during which the respective gene products are utilized.

Type
Research Article
Information
Genetics Research , Volume 43 , Issue 3 , June 1984 , pp. 257 - 275
Copyright
Copyright © Cambridge University Press 1984

References

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