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Effect of multiple copies of rpoBC on the rate of RNA synthesis in Escherichia coli

Published online by Cambridge University Press:  14 April 2009

Elena C. Guzman  and
Alfonso Jimenez-Sanchez
Elena C. Guzman
Affiliation:
Departamento de Genética, Universidad de Extremadura, 06071 Badajoz, Spain
Alfonso Jimenez-Sanchez
Affiliation:
Departamento de Genética, Universidad de Extremadura, 06071 Badajoz, Spain
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Summary

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The cloning of the rpoB and rpoC genes in a high copy number vector in E. coli increased the amount of the encoded gene products, the β and β′ subunits of RNA polymerase. However, this unexpectedly caused a 30–50% decrease in RNA synthetic activity which alternatively induced a reduction of growth rate and enlargement of cell size, and decreased the DNA replication time. The results can be explained by autogenous regulation of the RNA polymerase genes by the ββ′ subunits. A relation between the decrease in number of transcription units and the observed higher rate of movement of DNA replication forks is discussed.

Type
Research Article
Information
Genetics Research , Volume 48 , Issue 2 , October 1986 , pp. 61 - 64
Copyright
Copyright © Cambridge University Press 1986

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