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Direct determination of retrotransposon transposition rates in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Sergey V. Nuzhdin  and
Trudy F. C. Mackay
Sergey V. Nuzhdin*
Affiliation:
Department of Genetics, North Carolina State University, Raleigh, NC 27695–7614 and Institute of Molecular Genetics, Kurchatov Square 46, Moscow 123182, Russia
Trudy F. C. Mackay
Affiliation:
Department of Genetics, Box 7614, North Carolina State University, Raleigh NC 27695–7614
*
*Corresponding author.
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Summary

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Rates of transposition and excision of the Drosophila melanogaster retrotransposon elements mdg3, 297, Doc, roo and copia were estimated directly, by in situ hybridization analysis of their cytological insertion sites in 31 replicates of a highly inbred line that had accumulated spontaneous mutations for approximately 160generations. Estimated transposition rates of Doc, roo and copia were, respectively, 4·2 × 10−5, 3·1 × 10−3 and 1·3 − 10−3; no transpositions of 297 nor mdg3 were observed. Rates of transposition of copia varied significantly among sublines. Excisions were only observed for roo elements, at a rate of 9·0 × 10−6 per element per generation. Copy number averaged over these element families increased 5·9 %; therefore, in these lines the magnitude of the forces opposing transposable element multiplication were weaker than transposition rates.

Type
Short Paper
Information
Genetics Research , Volume 63 , Issue 2 , April 1994 , pp. 139 - 144
Copyright
Copyright © Cambridge University Press 1994

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