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A direct determination of nuclear distribution in heterokaryons of Aspergillus nidulans

Published online by Cambridge University Press:  14 April 2009

A. J. Clutterbuck  and
J. A. Roper
A. J. Clutterbuck
Affiliation:
Department of Genetics, University of Sheffield
J. A. Roper
Affiliation:
Department of Genetics, University of Sheffield
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A cytological study has been made, in Aspergillus nidulans, of haploid and diploid homokaryons and of heterokaryons formed between them. The effective region of growth was shown to extend about 600 μ behind the growing edge of the colony and to correspond largely with the tip cells. Acridine fluorescence gave unselective staining of all nuclei; projected nuclear areas, and their distributions in individual hyphae, were determined for the homokaryons and heterokaryons. Diploid and haploid nuclei differ in mean projected area but the overlap of their size distributions precluded unequivocal classification of individual nuclei in diploid–haploid heterokaryons. However, it was shown that the hyphae of a heterokaryon differ in nuclear ratios. This, and other evidence, strongly support the suggestion that heterokaryon maintenance and adaptation occur through interhyphal rather than intrahyphal selection.

Type
Research Article
Information
Genetics Research , Volume 7 , Issue 2 , April 1966 , pp. 185 - 194
Copyright
Copyright © Cambridge University Press 1966

References

REFERENCES

Buxton, E. W. (1954). Heterokaryosis and variability in Fusarium oxysporum f. solani (Snyder & Hansen). J. gen. Microbiol. 10, 71–84.Google Scholar
Clutterbuck, A. J. (1964). Heterokaryosis in Aspergillus nidulans. Ph.D. Thesis, University of Sheffield.Google Scholar
Clutterbuck, A. J. (1965). In preparation.Google Scholar
Jinks, J. L. (1952). Heterokaryosis: a system of adaptation in wild fungi. Proc. R. Soc. B, 140, 83–99.Google Scholar
Käfer, E. (1958). An 8-chromosome map of Aspergillus nidulans. Adv. Genet. 9, 105–145.CrossRefGoogle ScholarPubMed
Käfer, E. (1961). The processes of spontaneous recombination in vegetative nuclei of Aspergillus nidulans. Genetics, 46, 1581–1609.Google Scholar
Pittenger, T. H. & Atwood, K. C. (1956). Stability of nuclear proportions during growth of Neurospora heterokaryons. Genetics, 41, 227–241.Google Scholar
Pontecorvo, G. (1946). Genetic systems based on heterokaryosis. Cold Spring Harb. Symp. quant. Biol. 11, 193–201.Google Scholar
Pontecorvo, G., Roper, J. A., Hemmons, L. M., MacDonald, K. D. & Bufton, A. W. J. (1953). The genetics of Aspergillus nidulans. Adv. Genet. 5, 141–238.Google Scholar
Rees, H. & Jinks, J. L. (1952). The mechanism of variation in Penicillium heterokaryons. Proc. R. Soc. B, 140, 100–106.Google Scholar
Roper, J. A. (1952). Production of heterozygous diploids in filamentous fungi. Experientia, 8, 14–15.Google Scholar
Ryan, F. J., Beadle, G. W. & Tatum, E. L. (1943). The tube method of growth measurement of Neurospora. Am. J. Bot. 30, 784–799.CrossRefGoogle Scholar
Warr, J. R. & Roper, J. A. (1964). Resistance mutants of Aspergillus nidulans. Heredity, Lond. 19, 167.Google Scholar