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Derepression of F-lac in Salmonella typhimurium by a determinant for kanamycin resistance

Published online by Cambridge University Press:  14 April 2009

H. R. Smith ,
June N. Grindley ,
N. D. F. Grindley  and
E. S. Anderson
H. R. Smith
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9
June N. Grindley
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9
N. D. F. Grindley
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9
E. S. Anderson
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9
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When a determinant for neomycin-kanamycin resistance (K) was transferred by an F-lac factor into Salmonella typhimurium, the resulting KF-lac strain was sensitive to the male-specific phage μ2 and F-lac was derepressed. However, F-lac alone is repressed in S. typhimurium. When kanamycin resistance is spontaneously lost from S. typhimurium KF-lac an element persists which derepresses F-lac in S. typhimurium. The results are consistent with the hypothesis that a locus der, for derepression of F-lac in S. typhimurium, lies on the K plasmid. The R factor Rldrd19 is derepressed in K12 but is repressed in S. typhimurium. It also is derepressed by der. In contrast to F-lac and R1, another R factor, R136drdH8, is derepressed in both K12 and S. typhimurium, so that the intervention of der is unnecessary for its derepression in the salmonella host.

Type
Short Papers
Information
Genetics Research , Volume 16 , Issue 3 , December 1970 , pp. 349 - 353
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Anderson, E. S. (1965). A rapid screening test for transfer factors in drug sensitive Enterobacteriaceae. Nature, London 208, 1016–1017.CrossRefGoogle ScholarPubMed
Anderson, E. S. & Lewis, M. J. (1965 a). Drug resistance and its transfer in Salmonella typhimurium. Nature, London 206, 579–583.CrossRefGoogle ScholarPubMed
Anderson, E. S. & Lewis, M. J. (1965 b). Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium. Nature, London 208, 843–849.CrossRefGoogle ScholarPubMed
Anderson, E. S., Mayhew, J. N. & Grindley, N. D. F. (1969). Transfer of a neomycin-kanamycin resistance determinant by the F factor of Escherichia coli K-12. Nature, London 222, 349–351.CrossRefGoogle Scholar
Dettori, R., Maccacaro, G. A. & Piccinin, G. L. (1961). Sex specific bacteriophages of Escherichia coli K-12. Giornale di Microbiologia 9, 141–150.Google Scholar
Easterling, S. B., Johnson, E. M., Wohlhieter, J. A. & Baron, L. S. (1969). Nature of lactose-fermenting Salmonella strains obtained from clinical sources. Journal of Bacteriology 100, 35–41.CrossRefGoogle ScholarPubMed
Frydman, A. & Meynell, E. (1969). Interactions between de-repressed F-like R factors and wild type Colicin B factors: superinfection immunity and repressor susceptibility. Genetical Research 14, 315–322.CrossRefGoogle ScholarPubMed
Grindley, J. N., Grindley, N. D. F. & Anderson, E. S. (1970). Acridine treatment of F+ and Hfr strains of Escherichia coli K12 carrying a neomycin-kanamycin resistance determinant. Genetical Research 15, 327–334.CrossRefGoogle ScholarPubMed
Mäkelä, P. H., Lederberg, J. & Lederberg, E. M. (1962). Patterns of sexual recombination in enteric bacteria. Genetics 47, 1427–1439.CrossRefGoogle ScholarPubMed
Menyell, E. & Cooke, M. (1969). Repressor-minus and operator-constitutive de-repressed mutants of F-like R factors: their effects on chromosomal transfer by HfrC. Genetical Research 14, 309–313.CrossRefGoogle Scholar
Meynell, E. & Datta, N. (1967). Mutant drug resistant factors of high transmissibility. Nature, London 214, 885–887.CrossRefGoogle ScholarPubMed
Smith, C., Anderson, E. S. & Clowes, R. C. (1970). Stable composite molecular forms of an R factor. Bacteriological Proceedings 60–61.Google Scholar