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Deletion mapping of the niiA niaD gene region of Aspergillus nidulans

Published online by Cambridge University Press:  14 April 2009

A. Brian Tomsett  and
David J. Cove
A. Brian Tomsett
Affiliation:
Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, England
David J. Cove
Affiliation:
Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, England
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The genetic fine-structure of the niiA niaD gene region of Aspergillus nidulans has been studied using deletion mapping. Deletions were identified as niiA niaD double mutants and comprised 1% of spontaneous chlorate-resistant mutants. All such double mutants were shown to involve deletions and their frequency was not increased by mutagenic treatment with either N-methyl-N′-nitro-N-nitrosoguanidine or with ultraviolet light. Deletion maps of the niaD and niiA genes have been constructed. A further class of mutation was also mapped using the deletions. These crn mutations, which affect a gene whose function is as yet unknown, map on the centromere proximal side of niiA. This analysis of a eukaryote gene cluster will provide a framework upon which to base studies of the regulation of the nitrate assimilation pathway.

Type
Research Article
Information
Genetics Research , Volume 34 , Issue 1 , August 1979 , pp. 19 - 32
Copyright
Copyright © Cambridge University Press 1979

References

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