Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-08T05:34:00.149Z Has data issue: false hasContentIssue false
  • English
  • Français

Deletion mapping of the niiA niaD gene region of Aspergillus nidulans

Published online by Cambridge University Press:  14 April 2009

A. Brian Tomsett  and
David J. Cove
A. Brian Tomsett
Affiliation:
Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, England
David J. Cove
Affiliation:
Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, England
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The genetic fine-structure of the niiA niaD gene region of Aspergillus nidulans has been studied using deletion mapping. Deletions were identified as niiA niaD double mutants and comprised 1% of spontaneous chlorate-resistant mutants. All such double mutants were shown to involve deletions and their frequency was not increased by mutagenic treatment with either N-methyl-N′-nitro-N-nitrosoguanidine or with ultraviolet light. Deletion maps of the niaD and niiA genes have been constructed. A further class of mutation was also mapped using the deletions. These crn mutations, which affect a gene whose function is as yet unknown, map on the centromere proximal side of niiA. This analysis of a eukaryote gene cluster will provide a framework upon which to base studies of the regulation of the nitrate assimilation pathway.

Type
Research Article
Information
Genetics Research , Volume 34 , Issue 1 , August 1979 , pp. 19 - 32
Copyright
Copyright © Cambridge University Press 1979

References

REFERENCES

Åberg, B. (1947). On the mechanism of the toxic action of chlorate and some related substances upon young wheat plants. Kungliga Lantbrukshögskolans Annaler 15, 37–107.Google Scholar
Adelberg, E. A., Mandel, M. & Chen, G. C. C. (1965). Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli K12. Biochemical and biophysical research communications 18, 788–795.Google Scholar
Alderson, T. & Hartley, M. J. (1969). Specificity for spontaneous and induced forward mutation at several gene loci in Aspergillus nidulans. Mutation Research 8, 255–264.CrossRefGoogle ScholarPubMed
Arst, H. N. Jr. & Cove, D. J. (1973). Nitrate metabolite repression in Aspergillus nidulans. Molecular and general Genetics 126, 111–141.CrossRefGoogle ScholarPubMed
Benzer, S. (1959). On the topology of the genetic fine structure. Proceedings of the national Academy of Sciences, U.S.A. 45, 1607–1620.CrossRefGoogle ScholarPubMed
Benzer, S. (1961). On the topography of the genetic fine structure. Proceedings of the national Academy of Sciences, U.S.A. 47, 1025–1038.CrossRefGoogle ScholarPubMed
Clutterbuck, A. J. (1974). Aspergillus nidulans. In Handbook of Genetics (ed. King, R. C.). Vol. I. Bacteria, Bacteriophages and Fungi, pp. 447–510. New York, London: Plenum Press.Google Scholar
Clutterbuck, A. J. (1975). Ascospore maturation and germination. Aspergillus News Letter 13, 13.Google Scholar
Cove, D. J. (1966). The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochimica et Biophysica Acta 113, 51–56.CrossRefGoogle ScholarPubMed
Cove, D. J. (1967). Kinetic studies of the induction of nitrate reductase and cytochrome c reductase in the fungus Aspergillus nidulans. Biochemical Journal 104, 1033–1039.Google Scholar
Cove, D. J. (1970). Control of gene action in Aspergillus nidulans. Proceedings of the Royal Society of London B 176, 267–275.Google ScholarPubMed
Cove, D. J. (1976 a). Chlorate toxicity in Aspergillus nidulans: the selection and characterisation of chlorate resistant mutants. Heredity 36, 191–203.Google Scholar
Cove, D. J. (1976 b). Chlorate toxicity in Aspergillus nidulans: studies of mutants altered in nitrate assimilation. Molecular and general Genetics 146, 147–159.Google Scholar
Cove, D. J. (1979). Genetic studies of nitrate assimilation in Aspergillus nidulans. Biological Reviews (in the Press).Google Scholar
Cove, D. J. & Pateman, J. A. (1969). Autoregulation of the synthesis of nitrate reductase in Aspergillus nufulans. Journal of Bacteriology 97, 1374–1378.CrossRefGoogle Scholar
Hartley, M. J. (1969). Mutation in Aspergillus nidulans. Ph.D. thesis, University of Cambridge.Google Scholar
Kinghorn, J. R. & Pateman, J. A. (1975). Studies of partially repressed mutants at the tamA and areA loci in Aspergillus nidulans. Molecular and general Genetics 140, 137–147.Google Scholar
MacDonald, D. W. & Cove, D. J. (1974). Studies on temperature-sensitive mutants affecting the assimilatory nitrate reductase of Aspergillus nidulans. European Journal of Biochemistry 47, 107–110.CrossRefGoogle Scholar
Mackintosh, M. E. & Pritchard, R. M. (1963). The production and replica plating of micro colonies of Aspergillue nidulans. Genetical Research 4, 321–322.CrossRefGoogle Scholar
Pateman, J. A. & Cove, D. J. (1967). Regulation of nitrate reduction in Aspergillus nidulans. Nature 215, 1234–1237.CrossRefGoogle ScholarPubMed
Pateman, J. A., Cove, D. J., Rever, B. M. & Roberts, D. B. (1964). A common cofactor for nitrate reductase and xanthine dehydrogenase which also regulates the synthesis of nitrate reductase. Nature 201, 58.CrossRefGoogle ScholarPubMed
Pateman, J. A. & Kinghorn, J. R. (1975). Nitrogen metabolism. In Filamentous Fungi. vol. 2 (ed. Smith, J. E. and Berry, D.), pp. 159–237. London: Edward Arnold Press.Google Scholar
Pateman, J. A., Kinghorn, J. R., Dunn, E. & Forbes, E. (1973). Ammonium regulation in Aspergillus nidulans. Journal of Bacteriology 114, 943–950.CrossRefGoogle ScholarPubMed
Pateman, J. A., Rever, B. A. & Cove, D. J. (1967). Genetic and biochemical studies of nitrate reduction in Aspergillus nidulans. Biochemical Journal 104, 104–111.CrossRefGoogle ScholarPubMed
Pfeifer, D., Hirsch, M. J., Bergman, D. & Homlaoui, M. (1974). Non-random distribution of deletion endpoints in the gal operon of E. coli. Molecular and general Genetics 132, 203–213.CrossRefGoogle ScholarPubMed
Pontecorvo, G., Roper, J. A., Hemmons, L. M., MacDonald, K. D. & Bufton, A. W. J. (1953). The genetics of Aspergillus nidulane. Advances in Genetics 5, 141–238.Google Scholar
Rand, K. N. & Arst, H. N. Jr (1977). A mutation in Aspergillus nidulans which affects the regulation of nitrite reductase and is tightly linked to its structural gene. Molecular and general Genetics 155, 67–75.Google Scholar
Reif, H. J. & Saedler, M. (1975). ISI is involved in deletion formation in the gal region of E. coli K12. Molecular and general Genetics 137, 17–28.Google Scholar
Schmeisser, U., Ganem, D. & Miller, J. M. (1977). Genetic studies of the lac repressor. II. Fine structure deletion map of the lacI gene, and its correlation with the physical map. Journal of Molecular Biology 109, 303–326.Google Scholar