Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-24T07:52:05.787Z Has data issue: false hasContentIssue false
  • English
  • Français

The cytogenetic boundaries of the rDNA region within heterochromatin of the X chromosome of Drosophila melanogaster and their relation to male meiotic pairing sites

Published online by Cambridge University Press:  14 April 2009

R. Appels  and
A. J. Hilliker
R. Appels
Affiliation:
CSIRO, Division of Plant Industry, P.O. Box 1600, Canberra City, A.C.T. 2601, Australia
A. J. Hilliker
Affiliation:
CSIRO, Division of Plant Industry, P.O. Box 1600, Canberra City, A.C.T. 2601, Australia
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The proximal breakpoints of the inversion chromosomes In(1)ωm4 and In(1)m51b were shown, by in situ hybridization, to define the boundaries of the ribosomal DNA region located within the X chromosome heterochromatin (Xh). We estimate that at least 95% of the rDNA is located between the In(1)ωm4 and In(1)ωm51b proximal breakpoints. In contrast only 60–70% of the Type I intervening sequences located in Xh are located between these breakpoints. The Type I intervening sequences in the rDNA region occur as inserts in the 28S rRNA sequences while the remainder of the sequences are distal to the In(1)ωm4 breakpoint and not associated with rRNA genes.

The regions of Xh which contain rDNA and Type I intervening sequences were related to regions shown by Cooper (1964) to contribute to meiotic pairing between the X and Y chromosomes in male Drosophila. We demonstrate that the rRNA coding region contributes to X / Y pairing. However, no single region of Xh is required for fidelity of male meiotic pairing of the sex chromosomes.

Type
Research Article
Information
Genetics Research , Volume 39 , Issue 2 , April 1982 , pp. 149 - 156
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Appels, R., Steffensen, D. M. & Craig, S. (1979). A new method for mapping the threedimensional distribution of DNA sequences in nuclei. Experimental Cell Research 124, 436–441.CrossRefGoogle ScholarPubMed
Bridges, C. B. (1916). Non-disjunction as proof of the chromosome theory of heredity. Genetics 1, 1–52, 107–163.CrossRefGoogle ScholarPubMed
Cooper, K. W. (1964). Meiotic conjunctive elements not involving chiasmata. Proceedings of the National Academy of Science, U.S.A. 52, 1248–1255.CrossRefGoogle Scholar
Dawid, I. B. & Botchan, P. (1977). Sequences homologous to ribosomal insertions occur in the Drosophila genome outside the nucleolus organizer. Proceedings of the National Academy of Science, U.S.A. 74, 4233–4237.CrossRefGoogle ScholarPubMed
Gershenson, S. (1940). The nature of the so-called genetically inert parts of the chromosomes. Vid. Akad. Nauk. URSR, Kiev. 116pp. (in Ukranian). (English translation by Eugenia Krivshenka.)Google Scholar
Glover, D. M. & Hogness, D. S. (1977). A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster rDNA. Cell 10, 167–176.CrossRefGoogle Scholar
Hilliker, A. J., Appels, R. & Schalet, A. (1980). The genetic analysis of Drosophila melanogaster heterochromatin. Cell 21, 607–619.CrossRefGoogle Scholar
Lindsley, D. L. & Grell, E. H. (1968). Genetic variations of Drosophila melanogaster. Carnegie Institute of Washington Publication no. 627.Google Scholar
Lindsley, D. L. & Sandler, L. (1958). The meiotic behavior of grossly deleted X chromosomes in Drosophila melanogaster. Genetics 43, 547–563.CrossRefGoogle ScholarPubMed
Muller, H. J. & Painter, T. (1932). The differentiation of the sex chromosomes of Drosophila into genetically active and inert regions. Zeitschrift für induktive Abstammungs- Vererbungslehre 62, 316–365.Google Scholar
Peacock, W. J., Appels, R., Endow, S. & Glover, D. (1981). Chromosomal distribution of the major insert in Drosophila melanogaster 28S rRNA genes. Genetical Research 37, 209–214.CrossRefGoogle ScholarPubMed
Peacock, W. J. (1965). Non random segregation of chromosomes in Drosophila males. Genetics 51, 573–583.CrossRefGoogle Scholar
Peacock, W. J. & Miklos, G. L. G. (1973). Meiotic drive in Drosophila: New interpretations of the segregation disorder and sex chromosome systems. Advances in Genetics 17, 361–409.CrossRefGoogle Scholar
Ritossa, F. (1976). The bobbed locus. In The Genetics and Biology of Drosophila, Ib (ed. Ashburner, M. and Novitski, E.), pp. 801–846. London: Academic Press.Google Scholar
Sandler, L. & Braver, G. (1954). The meiotic loss of unpaired chromosomes in Drosophila melanogaster. Genetics 39, 365–377.CrossRefGoogle ScholarPubMed
Schalet, A. (1969). Exchanges at the bobbed locus of Drosophila melanogaster. Genetics 63, 133–153.CrossRefGoogle ScholarPubMed
Spear, B. B. (1974). The genes for ribosomal RNA in diploid and polytene chromosomes of Drosophila melanogaster. Chromosoma 48, 159–179.CrossRefGoogle ScholarPubMed
Steffensen, D. M., Appels, R. & Peacock, W. J. (1981). The chromosomal distribution of the 1·705 g/cc and 1·672 g/cc satellite DNA's in Drosophila melanogaster. Chromosoma 82, 525–541.CrossRefGoogle Scholar
Wellauer, P. K., Dawid, I. B. & Tartof, K. D. (1978). X and Y chromosomal ribosomal DNA of Drosophila. Comparison of spacers and insertions. Cell 14, 269–278.CrossRefGoogle ScholarPubMed
Yamamoto, M. & Miklos, G. L. G. (1977). Genetic dissection of heterochromatin in Drosophila: The role of basal X heterochromatin in meiotic sex chromosome behaviour. Chromosoma 60, 283–296.CrossRefGoogle ScholarPubMed