Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-28T00:25:26.592Z Has data issue: false hasContentIssue false
  • English
  • Français

Conventional and unconventional analysis of an inversion in Neurospora

Published online by Cambridge University Press:  14 April 2009

Barbara C. Turner  and
David D. Perkins
Barbara C. Turner
Affiliation:
Department of Biological Sciences, Stanford University, Stanford, California 94305, U.S.A.
David D. Perkins
Affiliation:
Department of Biological Sciences, Stanford University, Stanford, California 94305, U.S.A.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In(IL; IR)O Y348 is a pericentric inversion of linkage group I in N. crassa, with a breakpoint between fr and un-5 in the left arm and a breakpoint between ad-9 and nit-1 in the right arm. Approximate breakpoint location was found by tabulating crossovers between the rearrangement and markers in normal chromosome sequence. Inversion structure was verified by marked In O Y348 × In O Y348 crosses. Precise mapping of breakpoints was by duplication coverage. Inversions like O Y348 do not produce progeny with segmental chromosome duplications when crossed to normal sequence, but duplications were produced by crossing it to In(IL; IR)O Y323 (Barry & Leslie, 1982), another standard pericentric inversion, and to T(I → VI)NM103 (Turner, 1977), a translocation to a tip. Each of these rearrangements has a breakpoint within the inverted region of In O Y348. Two duplications from In O Y348 × In O Y323 were converted to normal chromosome sequence by double mitotic recombination. Besides expediting mapping, the technique of intercrossing rearrangements increasingly enables us to make segmental duplications exactly tailored for studying specific included genes.

Type
Research Article
Information
Genetics Research , Volume 40 , Issue 2 , October 1982 , pp. 175 - 190
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Barry, E. G. & Leslie, J. F. (1982). An interstitial pericentric inversion in Neurospora. Canadian Journal of Genetics and Cytology. (Submitted.)CrossRefGoogle Scholar
Birchler, J. A. (1980). The cytological localization of the alcohol dehydrogenase-1 locus in maize. Genetics 94, 687–700.CrossRefGoogle ScholarPubMed
Lindsley, D. L., Sandler, L., Baker, B. S., Carpenter, A. T. C., Denell, R. E., Hall, J. C., Jacobs, P. A., Miklos, G. L. G., Davis, B. K., Gethmann, R. C., Hardy, R. W., Hessler, A., Miller, S. M., Nozawa, H., Parry, D. M. & Gould-Somero, M. (1972). Segmental aneuploidy and the genetic gross structure of the Drosophila genome. Genetics 71, 157–184.CrossRefGoogle ScholarPubMed
Metzenberg, R. L. & Chia, W. (1979). Genetic control of phosphorus assimilation in Neurospora crassa: dose-dependent dominance and recessiveness in constitutive mutants. Genetics 93, 625–643.CrossRefGoogle ScholarPubMed
Newmeyer, D. & Galeazzi, D. R. (1977). The instability of Neurospora duplication Dp(IL → IR)H4250, and its genetic control. Genetics 85, 461–487.CrossRefGoogle ScholarPubMed
Newmeyer, D. & Taylor, C. W. (1967). A pericentric inversion in Neurospora, with unstable duplication progeny. Genetics 56, 771–791.CrossRefGoogle ScholarPubMed
Perkins, D. D. (1972). An insertional translocation that generates duplications heterozygous for mating type. Genetics 71, 25–51.CrossRefGoogle ScholarPubMed
Perkins, D. D. (1974). The manifestation of chromosome rearrangements in unordered asci of Neurospora. Genetics 77, 459–489.CrossRefGoogle ScholarPubMed
Perkins, D. D. & Barry, E. G. (1977). The cytogenetics of Neurospora. Advances in Genetics 19, 133–285.CrossRefGoogle ScholarPubMed
Perkins, D. D., Newmeyer, D., Taylor, C. W. & Bennett, D. C. (1969). New markers and map sequences in Neurospora crassa with a description of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetics 40, 247–278.Google ScholarPubMed
Perkins, D. D., Newmeyer, D. & Turner, B. C. (1972). Nontandem duplications in Neurospora, and restoration of the euploid condition by chromosome breakage. Genetics 71, s46–s47 (abstr.).Google Scholar
Perkins, D. D., Raju, N. B. & Barry, E. G. (1980). A chromosome rearrangement in Neurospora that produces viable progeny containing two nucleolus organizers. Chromosoma 76, 255–275.CrossRefGoogle ScholarPubMed
Rhoades, M. M. & Dempsey, E. (1956). Studies with overlapping inversions. Maize Genetics Cooperative News Letter 30, 42–47.Google Scholar
Rodland, K. D. & Russell, P. J. (1982). Regulation of ribosomal RNA cistron number in a strain of Neurospora crassa with a duplication of the nucleolus organizer region. Biochimica et Biophysica Acta. (In the Press.)CrossRefGoogle Scholar
Smith, D. A. (1974). Unstable diploids of Neurospora and a model for their somatic behavior. Genetics 76, 1–17.CrossRefGoogle Scholar
Sturtevant, A. H. & Beadle, G. W. (1936). The relations of inversions in the X chromosome of Drosophila melanogaster to crossing over and disjunction. Genetics 21, 554–604.CrossRefGoogle ScholarPubMed
Turner, B. C. (1976). Dominance of the wild-type (sensitive) allele of cyh-1. Neurospora Newsletter 23, 24.Google Scholar
Turner, B. C. (1977). Euploid derivatives of duplications from a translocation in Neurospora. Genetics 85, 439–460.CrossRefGoogle ScholarPubMed
Turner, B. C., Taylor, C. W., Perkins, D. D. & Newmeyer, D. (1969). New duplication-generating inversions in Neurospora. Canadian Journal of Genetics and Cytology 11, 622–638.CrossRefGoogle ScholarPubMed
Westergaard, M. & Mitchell, H. K. (1947). Neurospora. V. A synthetic medium favoring sexual reproduction. American Journal of Botany 34, 573–577.CrossRefGoogle Scholar
Yoder, O. C. (1979). Experience with the Applegate–Nelson–Metzenberg method of enrichment in high sorbose medium. Neurospora Newsletter 26, 23–24.Google Scholar