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Constitutive magnification by the Ybb− chromosome of Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Donald J. Komma ,
Susan J. Glass  and
Sharyn A. Endow
Donald J. Komma
Affiliation:
Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
Susan J. Glass
Affiliation:
Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
Sharyn A. Endow
Affiliation:
Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
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Ybb− is an rDNA-deficient chromosome of Drosophila that has often been used in magnification experiments to induce high-frequency reversion of bobbed (bb) chromosomes. We observed previously that Ybb− causes ring chromosome loss even when the rings are bb+, suggesting that Ybb− induces magnifying sister chromatid exchanges in bb+ rings. Here we show that the Ybb- chromosome causes low levels of bb magnification in bb+ flies. We refer to the ability of Ybb− to bypass the rDNA deficiency requirement for magnification as ‘constitutive’ magnification. We have magnified the ribosomal genes on the Ybb− chromosome and analysed the revertant chromosomes using genetic and molecular methods. We find that magnified Ybb− chromosomes also exhibit constitutive magnifier activity. Molecular analysis shows that both type 1 and type 2 intron+ ribosomal gene repeats are associated with magnified Ybb− chromosomes. Type 2 introns have been described previously in the rDNA of both X and Y chromosomes. However, type 1 intervening sequences are thought to be present only in X, but not Y, ribosomal genes. Some of the Ybb− type 1 insertions differ from those present in the rDNA of X chromosomes in that they contain an EcoRl site, and some may be present in tandem arrays. The constitutive magnifier activity of Ybb− may reside either in the structurally unusual ribosomal gene intervening sequences associated with the chromosome, or in the locus on YL that is required for magnification to occur.

Type
Research Article
Information
Genetics Research , Volume 62 , Issue 3 , December 1993 , pp. 205 - 212
Copyright
Copyright © Cambridge University Press 1993

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