Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-10T20:37:42.505Z Has data issue: false hasContentIssue false
  • English
  • Français

Conditional mutations affecting the cell envelope of Escherichia coli K-12

Published online by Cambridge University Press:  14 April 2009

A. F. Egan  and
R. R. B. Russell
A. F. Egan
Affiliation:
CSIRO Division of Food Research, Meat Research Laboratory, Cannon Hill, Queensland, Australia4170
R. R. B. Russell
Affiliation:
CSIRO Division of Food Research, Meat Research Laboratory, Cannon Hill, Queensland, Australia4170
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Amongst a collection of temperature sensitive (TS) mutants of Escherichia coli K-12, some have been found which can grow at the restrictive temperature (42 °C) if the osmotic pressure of the medium is raised by the addition of sodium chloride (1%) or sucrose (12·5%). These mutants are described as temperature sensitive osmotic remedial (TSOR) mutants. At the restrictive temperature they are not osmotically fragile, but do display decreased resistance to inhibitory agents such as deoxycholate, actino-mycin D and acridine orange; they also show release of the periplasmic enzyme ribonuclease. These results indicate a change in the cell's outer permeability barrier. The genes affected in six of the mutants have been located on the E. coli linkage map. The mutations, which occur at loci not previously described, have been named envM–envT to indicate their effect on the cell envelope.

Type
Research Article
Information
Genetics Research , Volume 21 , Issue 2 , April 1973 , pp. 139 - 152
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Adeiberg, E. A. & Burns, S. N. (1960). Genetic variation in sex factor of Escherichia coli. Journal of Bacteriology 79, 321–330.CrossRefGoogle Scholar
Bukhari, A. I. & Taylor, A. L. (1971). Genetic analysis of diamino-pimelic acid- and lysine-requiring mutants of Escherichia coli. Journal of Bacteriology 105, 844–854.CrossRefGoogle Scholar
Dowman, J. E. & Meynell, G. G. (1970). Pleiotropic effects of derepressed bacterial sex factors on colicinogeny and cell wall structure. Molecular and General Genetics 109, 57–68.CrossRefGoogle ScholarPubMed
Ennis, H. L. (1971). Mutants of Escherichia coli sensitive to antibiotics. Journal of Bacteriology 107, 486–490.CrossRefGoogle ScholarPubMed
Eriksson-Grennberg, K. G., Nordström, K. & Englund, P. (1971). Resistance of Escherichia coli to penicillins. IX. Genetics and physiology of Class II Ampicillin-resistant mutants that are galactose negative or sensitive to Bacteriophage C21, or both. Journal of Bacteriology 108, 1210–1223.CrossRefGoogle ScholarPubMed
Hambleton, P. (1971). Repair of wall damage in Escherichia coli recovered from an aerosol. Journal of General Microbiology 69, 81–88.CrossRefGoogle ScholarPubMed
Higa, A. & Mandel, M. (1970). Actinomycin sensitive mutants of Escherichia coli K-12. Molecular and General Genetics 108, 41–46.CrossRefGoogle ScholarPubMed
Htrota, Y., Wakil, S., Shapiro, B., Ryter, A., Hubwitz, J. & Jacob, F. (1969). Sur un mutant thermosensible d'Escherichia coli presentant des anomalies de la membrane. Comptes Rendus Academie des Sciences, Paris, Série D 269, 1346–1348.Google Scholar
Holland, I. B., Threlfall, E. J., Holland, E. M., Dabby, V. & Samson, A. C. R. (1970). Mutants of Escherichia coli with altered surface properties which are refractory to Colicin E2, sensitive to ultraviolet light and which can also show recombination deficiency, abortive growth of Bacteriophage λ and filament formation. Journal of General Microbiology 62, 371–382.CrossRefGoogle ScholarPubMed
Im, S. W. K., Davidson, H. & Pittard, J. (1971). Phenylalanine and tyrosine biosynthesis in Escherichia coli K-12: mutants derepressed for 3-deoxy-D-arabinoheptulosonic acid 7-phosphate synthetase (phe), 3-deoxy-D-arabinoheptulosonic acid 7-phosphate synthetase (tyr), chorismate mutase T-prephenate dehydrogenase and transaminase A. Journal of Bacteriology 108, 400–409.CrossRefGoogle ScholarPubMed
Imae, Y. (1968). Mitomycin C-sensitive mutant of Escherichia coli K12. Journal of Bacteriology 95, 1191–1192.CrossRefGoogle Scholar
Letve, L. (1968). Studies on the permeability change produced in coliform bacteria by ethy-lenediaminetetraacetate. Journal of Biological Chemistry 243, 2373–2380.Google Scholar
Lopes, J., Gottfried, S. & Rothfield, L. (1972). Leakage of periplasmic enzymes by mutants of Escherichia coli and Salmonella typhimurium; Isolation of ‘periplasmic leaky’ mutants. Journal of Bacteriology 109, 520–525.CrossRefGoogle ScholarPubMed
Lugtenberg, E. J. J., de Haas-Menger, L. & Ruyters, W. H. M. (1972). Murein synthesis and identification of cell wall precursors of temperature-sensitive lysis mutants of Escherichia coli. Journal of Bacteriology 109, 326–335.CrossRefGoogle ScholarPubMed
Makë;lä, P. H. & Stocker, B. A. D. (1969). Genetics of polysaccharide biosynthesis. Annual Review of Genetics 3, 291–322.CrossRefGoogle Scholar
Mangiarotti, G., Apirion, D. & Schlessinger, D. (1966). Selection of sucrose-dependent Escherichia coli to obtain envelope mutants and fragile cultures. Science 153, 892–894.CrossRefGoogle ScholarPubMed
Matsuzawa, H., Matsuhashi, M., Oka, A. & Sugino, Y. (1969). Genetic and biochemical studies on cell wall peptidoglycan synthesis in Escherichia coli K-12. Biochemical and Biophysical Research Communications 36, 682–689.CrossRefGoogle ScholarPubMed
Matzura, H. & Broda, P. (1968). Sensitization of Escherichia coli to actinomycin D by the arrest of protein synthesis. Journal of Bacteriology 96, 1877–1879.CrossRefGoogle ScholarPubMed
Monod, J., Cohen-Bazibe, G. & Cohn, M. (1951). Sur la biosynthése de la β-galactosidase (lactase) chez Escherichia coli. La spécificité de l'induction. Biochimica et Biophysica Acta 7, 585–599.CrossRefGoogle Scholar
Muschel, L. H. & Gustafson, L. (1968). Antibotic, detergent and complement sensitivity of Salmonella typhi after ethylenediaminetetraacetic acid treatment. Journal of Bacteriology 95, 2010–2013.CrossRefGoogle Scholar
Nagel de Zwaig, R. & Luria, S. E. (1967). Genetics and physiology of colicin-tolerant mutants of Escherichia coli. Journal of Bacteriology 94, 1112–1123.CrossRefGoogle ScholarPubMed
Nakamura, H. (1968). Genetic dtermination of resistance to acriflavine, phenethyl alcohol and sodium dodecylsulfate in Escherichia coli. Journal of Bacteriology 96, 987–996.CrossRefGoogle Scholar
Normark, S. (1969). Mutation in Escherichia coli K-12 mediating spherelike envelopes and changed tolerance to ultraviolet irradiation and some antibiotics. Journal of Bacteriology 98, 1274–1277.CrossRefGoogle ScholarPubMed
Normark, S. (1970). Genetics of a chain-forming mutant of Escherichia coli. Transduction and dominance of the envA gene mediating increased penetration to some antibacterial agents. Genetical Research 16, 63–78.CrossRefGoogle ScholarPubMed
Nozawa, R. & Mizuno, D. (1968). Isolation and properties of an aetinomycin sensitive mutant of Escherichia coli. Journal of Biochemistry 63, 795–797.CrossRefGoogle ScholarPubMed
Otsuji, N. & Higashi, T. (1970). Properties of mitomycin ‘C’ sensitive mutants of Escherichia coli. Japanese Journal of Genetics 45, 493.Google Scholar
Ray, B., Jezeski, J. J. & Busta, F. F. (1971). Repair of injury in freeze-dried Salmonella anatum. Applied Microbiology 22, 401–407.CrossRefGoogle ScholarPubMed
Ricard, M., Hirota, Y. & Jacob, F. (1970). Isolement de mutants de membrane chez Escherichia coli. Comptes Rendus Academie des Sciences, Paris, Série D 270, 2591–2593.Google ScholarPubMed
Romero, E., Riva, S., Fietta, A. M. & Silvestri, L. G. (1971). Effect of R factors on rifampicin resistance in E. coli. Nature New Biology 234, 56–58.CrossRefGoogle ScholarPubMed
Russell, R. R. B. (1972). Temperature sensitive osmotic remedial mutants of Escherichia coli. Journal of Bacteriology 112, 661.CrossRefGoogle ScholarPubMed
Russell, R. R. B. & Pittard, A. J. (1971). Mutants of Escherichia coli unable to make protein at 42C. Journal of Bacteriology 108, 790–798.CrossRefGoogle Scholar
Schlief, R. (1969). Isolation and characterization of a streptolydigin resistant RNA polymerase. Nature, London 223, 1068–1069.CrossRefGoogle Scholar
Sekiguchi, M. & Iida, S. (1967). Mutants of Escherichia coli permeable to aetinomycin. Proceedings of the National Academy of Sciences of the United States of America 58, 2315–2320.CrossRefGoogle Scholar
Sugino, Y. (1966). Mutants of Escherichia coli sensitive to methylene blue and acridines. Genetical Research 7, 1–11.CrossRefGoogle ScholarPubMed
Taketo, A., Hayashi, A. & Kuno, S. (1972). Sensitivity of Escherichia coli to viral nucleic acid. IV. Transfection of phage DNA to mutant thermosensitive in wall synthesis. Journal of Biochemistry 71, 513–518.Google ScholarPubMed
Tamaki, S., Sato, T. & Matsuhashi, M. (1971). Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12. Journal of Bacteriology 105, 968–975.CrossRefGoogle ScholarPubMed
Taylor, A. L. (1970). Current Linkage Map of Escherichia coli. Bacteriological Reviews 34, 155–175.CrossRefGoogle ScholarPubMed
Whitney, E. N. (1971). The tolC locus in Escherichia coli K12. Genetics 67, 39–53.CrossRefGoogle ScholarPubMed
Wijsman, H. J. W. (1972). Mutation affecting plasmolysis in Escherichia coli. Journal of Bacteriology 110, 789–790.CrossRefGoogle ScholarPubMed
Wilkinson, R. G., Gemski, P. & Stocker, B. A. D. (1972). Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping. Journal of General Microbiology 70, 527–554.CrossRefGoogle ScholarPubMed
Willetts, N. S., Clark, A. J. & Low, B. (1969). Genetic location of certain mutations conferring recombination deficiency in Escherichia coli. Journal of Bacteriology 97, 244–249.CrossRefGoogle ScholarPubMed