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Conditional mutations affecting the cell envelope of Escherichia coli K-12

Published online by Cambridge University Press:  14 April 2009

A. F. Egan  and
R. R. B. Russell
A. F. Egan
Affiliation:
CSIRO Division of Food Research, Meat Research Laboratory, Cannon Hill, Queensland, Australia4170
R. R. B. Russell
Affiliation:
CSIRO Division of Food Research, Meat Research Laboratory, Cannon Hill, Queensland, Australia4170
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Summary

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Amongst a collection of temperature sensitive (TS) mutants of Escherichia coli K-12, some have been found which can grow at the restrictive temperature (42 °C) if the osmotic pressure of the medium is raised by the addition of sodium chloride (1%) or sucrose (12·5%). These mutants are described as temperature sensitive osmotic remedial (TSOR) mutants. At the restrictive temperature they are not osmotically fragile, but do display decreased resistance to inhibitory agents such as deoxycholate, actino-mycin D and acridine orange; they also show release of the periplasmic enzyme ribonuclease. These results indicate a change in the cell's outer permeability barrier. The genes affected in six of the mutants have been located on the E. coli linkage map. The mutations, which occur at loci not previously described, have been named envM–envT to indicate their effect on the cell envelope.

Type
Research Article
Information
Genetics Research , Volume 21 , Issue 2 , April 1973 , pp. 139 - 152
Copyright
Copyright © Cambridge University Press 1973

References

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