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The comparison of closely related groups of rodents by DNA/DNA annealing

Published online by Cambridge University Press:  14 April 2009

Anne McLaren
Affiliation:
A.R.G. Unit of Animal Genetics, Institute of Animal Genetics, Edinburgh
P. M. B. Walker
Affiliation:
Department of Zoology, Edinburgh University
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Within the genus Apodemus, DNA from A. sylvaticus and A. flavicollis could be distinguished on the basis of reciprocal DNA/DNA annealing in agar, even when unfractionated DNA was used. In preliminary experiments, DNA from geographical races of A. sylvaticus could not be distinguished, nor could DNA from two closely related species of Peromyscus.

Within the species Mus musvulus, the fast-renaturing fraction of L-cell DNA, separated on a hydroxyapatite column, was able to discriminate between L-cell (originating from the C3H inbred strain) and NZB (New Zealand Black) inbred strain DNA in agar.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1968

References

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