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Codon usage as a reason for unsuccessful search for amber-suppressor mutants in Streptomyces lividans?

Published online by Cambridge University Press:  14 April 2009

Horst Schmieger ,
Ralph Knerr  and
Maria Köberlein
Horst Schmieger*
Affiliation:
Institut für Genetik und Mikrobiologie, Lehrstuhl Genetik, Maria-Ward-Str. la, D-8000 München 19, FRG
Ralph Knerr
Affiliation:
Institut für Genetik und Mikrobiologie, Lehrstuhl Genetik, Maria-Ward-Str. la, D-8000 München 19, FRG
Maria Köberlein
Affiliation:
Institut für Genetik und Mikrobiologie, Lehrstuhl Genetik, Maria-Ward-Str. la, D-8000 München 19, FRG
*
* Corresponding author
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Summary

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An amber mutation was created in the CAT gene of plasmid vector pACYC184 and this modified plasmid was fused with the Streptomyces vector pIJ702 for use as an indicator for the identification of Streptomyces strains carrying nonsense suppressor tRNA mutations. The resulting hybrid plasmid pGM1109 was introduced into the chloramphenicol-sensitive mutant M252 of Streptomyces lividans. Chloramphenicol-resistant colonies were isolated and characterized. None of them was a nonsense suppressor mutant. The failure to obtain such mutants is discussed on the basis of codon usage in streptomycetes.

Type
Research Article
Information
Genetics Research , Volume 52 , Issue 3 , December 1988 , pp. 163 - 168
Copyright
Copyright © Cambridge University Press 1988

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