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The clock gene period in the medfly Ceratitis capitata

Published online by Cambridge University Press:  26 September 2005

GABRIELLA M. MAZZOTTA ,
FEDERICA SANDRELLI ,
MAURO A. ZORDAN ,
MOYRA MASON ,
CLARA BENNA ,
PAOLA CISOTTO ,
EZIO ROSATO ,
CHARALAMBOS P. KYRIACOU  and
RODOLFO COSTA
GABRIELLA M. MAZZOTTA
Affiliation:
Department of Biology, University of Padua, via U. Bassi, 58/B, 35131 Padua, Italy
FEDERICA SANDRELLI
Affiliation:
Department of Biology, University of Padua, via U. Bassi, 58/B, 35131 Padua, Italy
MAURO A. ZORDAN
Affiliation:
Department of Biology, University of Padua, via U. Bassi, 58/B, 35131 Padua, Italy
MOYRA MASON
Affiliation:
Department of Biology, University of Padua, via U. Bassi, 58/B, 35131 Padua, Italy
CLARA BENNA
Affiliation:
Department of Biology, University of Padua, via U. Bassi, 58/B, 35131 Padua, Italy
PAOLA CISOTTO
Affiliation:
Department of Biology, University of Padua, via U. Bassi, 58/B, 35131 Padua, Italy
EZIO ROSATO
Affiliation:
Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, UK
CHARALAMBOS P. KYRIACOU
Affiliation:
Department of Genetics, University of Leicester, University Road, Leicester, LE1 7RH, UK
RODOLFO COSTA
Affiliation:
Department of Biology, University of Padua, via U. Bassi, 58/B, 35131 Padua, Italy
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Abstract

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We have isolated the clock gene period (per) from the medfly Ceratitis capitata, one of the most economically important insect pest species. The overall pattern of conserved, non-conserved and functional domains that are observed within dipteran and lepidopteran per orthologues is preserved within the coding sequence. Expression analysis from fly heads revealed a daily oscillation in per mRNA in both light[ratio ]dark cycles and in constant darkness. However PER protein levels from head extracts did not show any significant evidence for cycling in either of these two conditions. When the Ceratitis per transgene under the control of the Drosophila per promoter and 3′UTR was introduced into Drosophila per-null mutant hosts, the transformants revealed a low level of rescue of behavioural rhythmicity. Nevertheless, the behaviour of the rhythmic transformants showed some similarities to that of Ceratitis, suggesting that Ceratitis per carries species-specific information that can evidently affect the Drosophila host's downstream rhythmic behaviour.

Type
Research Article
Information
Genetics Research , Volume 86 , Issue 1 , August 2005 , pp. 13 - 30
Copyright
© 2005 Cambridge University Press