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Chromosomal fragment responsible for genetic mosaicism in larval body marking of the silkworm, Bombyx mori

Published online by Cambridge University Press:  14 April 2009

Haruhiko Fujiwara ,
Osamu Ninaki ,
Masahiko Kobayashi ,
Jun Kusuda  and
Hideaki Maekawa
Haruhiko Fujiwara*
Affiliation:
Department of Technology, National Institute of Health, Kamiosaki, Shinagawa-ku, Tokyo 141, Japan
Osamu Ninaki
Affiliation:
Department of Insect Genetics and Breeding, National Institute of Sericultural and Entomological Science, Kobuchizawa, Yamanashi 409–16, Japan
Masahiko Kobayashi
Affiliation:
Laboratory of Sericultural Sciences, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
Jun Kusuda
Affiliation:
Department of Virology and Rickettsiology, National Institute of Health, Kamiosaki, Shinagawa-ku, Tokyo 141, Japan
Hideaki Maekawa
Affiliation:
Department of Technology, National Institute of Health, Kamiosaki, Shinagawa-ku, Tokyo 141, Japan
*
* Corresponding author.
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Several genetic mosaics for larval body marking of the silkworm, Bombyx mori, have been induced by X-ray irradiation. It is hypothesized that the occasional loss of chromosomal fragments carrying the genes for body marking during development may give rise to this type of mosaicism. Using pulsed field gel electrophoresis (PFGE), we find that a DNA molecule of about 2·5 megabases (Mb) is present in one type of mosaic (mottled striped strains pSm788 and pSm872), and not in any other strain. This DNA fragment hybridizes strongly with some chorion genes which are less than 6·9 cM away from the ps locus, and hence it corresponds to a chromosomal fragment containing genes for both striped marking (ps) and the chorion. In the non-mottled ps strain, the phenotype before X-ray irradiation, no band was detected either on a PFGE gel or after hybridization with the chorion probe. These results suggest that the mottled ps strains carry short chromosome fragments which are lost differentially during cell divisions.

Type
Research Article
Information
Genetics Research , Volume 57 , Issue 1 , February 1991 , pp. 11 - 16
Copyright
Copyright © Cambridge University Press 1991

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