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Choline-O-sulphate utilization in Aspergillus nidulans

Published online by Cambridge University Press:  14 April 2009

Roy A. Gravel
Roy A. Gravel
Affiliation:
Department of Biology, Kline Biology Tower, Yale University, New Haven, Connecticut 06520
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The role of choline-O-sulphate (COS) as a sulphur storage compound in Aspergillus nidulans was examined by comparing a normal strain and one unable to utilize COS in a sulphur-starvation experiment designed to measure the mobilization of sulphur stores. Efforts to isolate the necessary mutants deficient in choline sulphatase activity produced two nutritionally distinct classes of mutants unable to utilize COS. They were found to be allelic on the basis of genetic complementation and fine structures mapping and represent either leaky or tight mutants with respect to choline sulphatase activity. One of these mutants with no detectable choline sulphatase activity was selected for a growth experiment which demonstrated that COS is a major, though not the only source of the endogenous sulphur supply which can be mobilized during growth in sulphur-limiting conditions.

Type
Research Article
Information
Genetics Research , Volume 28 , Issue 3 , December 1976 , pp. 261 - 276
Copyright
Copyright © Cambridge University Press 1976

References

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