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Chloroplast DNA diversity in wild and cultivated barley: implications for genetic conservation

Published online by Cambridge University Press:  14 April 2009

M. T. Clegg ,
A. H. D. Brown  and
P. R. Whitfeld
M. T. Clegg
Affiliation:
Departments of Botany and Molecular and Population Genetics, University of Georgia, Athens, GA 30602
A. H. D. Brown
Affiliation:
CSIRO, Division of Plant Industry, Canberra City, ACT 2601, Australia
P. R. Whitfeld
Affiliation:
CSIRO, Division of Plant Industry, Canberra City, ACT 2601, Australia
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Summary

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Nine diverse lines of cultivated barley (Hordeum vulgare) and 11 lines of its wild progenitor (H. spontaneum) were assayed for variation in their chloroplast DNA by digestion with ten restriction endonucleases. The cultivated lines exhibited a single cpDNA polymorphism, whereas the wild material exhibited five. The significantly lower level of diversity among the cultivated lines was unexpected because both cultivated and wild lines had been selected for comparable levels of diversity for nuclear encoded isozyme loci. These results suggest that the level of cytoplasmic diversity was markedly restricted during the domestication of cultivated barley.

Type
Short Paper
Information
Genetics Research , Volume 43 , Issue 3 , June 1984 , pp. 339 - 343
Copyright
Copyright © Cambridge University Press 1984

References

REFERENCES

Avise, J. C., Gibblin-Davidson, C., Laerm, J., Patton, J. C. & Lansman, R. A. (1979). Mitochondrial DNA clones and matriarchal phylogeny within and among geographic populations of the pocket gopher, Geomys pinetis. Proceedings of the National Academy of Sciences, USA 76, 6694–6698.CrossRefGoogle ScholarPubMed
Bowman, C. M. & Dyer, T. A. (1982). Purification and analysis of DNA from wheat chloroplasts isolated in nonaqueous media. Analytical Biochemistry 122, 108–118.CrossRefGoogle ScholarPubMed
Brown, A. H. D. & Clegg, M. T. (1983). Isozyme assessment of plant genetic resources. In, Isozyme Current Topics in Biological and Medical Research. Vol. II. Medical and Other Applications (ed. Rattazzi, M. C., Scandalios, J. G. and Whitt, G. S.), pp. 285–295. New York: Alan R. Liss, Inc.Google Scholar
Brown, A. H. D. & Munday, J. (1982). Population genetic structure and optimal sampling of land races of barley from Iran. Oenetica 58, 85–95.Google Scholar
Clegg, M. T., Rawson, J. R. Y. & Thomas, K. (1984). Chloroplast DNA variation in pearl millet and related species. Genetics 106, 449–461.CrossRefGoogle ScholarPubMed
Doll, H. & Brown, A. H. D. (1979). Hordein variation in wild (Hordeum spontaneum) and cultivated (H. vulgare) barley. Canadian Journal of Genetics and Cytology 21, 391–404.CrossRefGoogle Scholar
Nevo, E., Zohary, D., Brown, A. H. D. & Haber, M. (1979). Genetic diversity and environmental associations of wild barley, Hordeum spontaneum, in Israel. Evolution 33, 815–833.Google ScholarPubMed
Palmer, J. D. & Zamir, D. (1982). Chloroplast DNA evolution and phylogenetic relationships in Lycopersicon. Proceedings of the National Academy of Sciences, USA 79, 5006–5010.CrossRefGoogle ScholarPubMed
Pring, D. R. & Levings, C. S. (1978). Heterogeneity of maize cytoplasmic genomes among male-sterile cytoplasms. Genetics 89, 121–136.CrossRefGoogle ScholarPubMed
Rick, C. M. & Fobes, J. F. (1975). Allozyme variation in the cultivated tomato. Bulletin of the Torrey Botanical Club 102, 376–384.CrossRefGoogle Scholar
Timothy, D. H., Levings, C. S., Pring, D. R., Conde, M. F. & Kernicke, J. L. (1979). Organelle DNA variation and systematic relationships in the genus Zea; Teosinte. Proceedings of the National Academy of Sciences, USA 76, 4220–4224.CrossRefGoogle ScholarPubMed
Weissinger, A. K., Timothy, D. H., Levings, C. S., Hu, W. W. L. & Goodman, M. M. (1982). Unique plasmid-like mitochondria! DNAs from indigenous maize races of Latin America. Proceedings of the National Academy of Sciences, USA 79, 1–5.CrossRefGoogle Scholar