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Characterization of the new osmotic mutants (os) which originated during genetic transformation in Neurospora crassa

Published online by Cambridge University Press:  14 April 2009

N. C. Mishra
N. C. Mishra
Affiliation:
Genetics Laboratory, Department of Biology, University of South Carolina, Columbia, S. C. 29208
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Inositol independent (inl+) strains were obtained either as transformants following treatment of the inositol requiring (inl) strains of Neurospora crassa with the wild-type DNA or as revertants without any DNA treatment. A significant number of the inositol-independent transformants were also found to have acquired additional mutations called osmotics (os) which made them unable to grow on 1 m-NaCl medium. None of the inositol-independent revertants were found to possess such osmotic mutations and their growth remained unaffected by the presence of NaCl. Many of the osmotic mutants described here were found to be new alleles of the previously known os–1 mutation on the linkage group I of Neurospora crassa. The remainder were found to map at two new genetic loci designated as os-6 and os-7; these loci were found to be closely linked to os–1. Among the new osmotic mutants only os-1 and os-6 mutants showed intragenic complementation. The mechanism of DNA-induced mutation during transformation is discussed.

Type
Research Article
Information
Genetics Research , Volume 29 , Issue 1 , February 1977 , pp. 9 - 19
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Bukhari, A. & Zipser, D. (1972). Random insertion of Mu-1 DNA within a single gene. Nature New Biology 236, 240–243.CrossRefGoogle Scholar
Bukhari, A. & Taylor, A. L. (1975). Influence of insertions on packaging of host sequences covalently linked to bacteriophage Mu DNA. Proceedings of the National Academy of Sciences of the United States of America, 72, 4399–4403.CrossRefGoogle ScholarPubMed
Crick, F. H. C., Barnett, L., Brenner, S. & Walls-Tobin, R. J. (1961). General nature of the genetic code for proteins. Nature 192, 1227–1232.CrossRefGoogle ScholarPubMed
Demerec, M. (1963). Selfer mutants of Salmonella typhimurium. Genetics 48, 1519–1531.CrossRefGoogle ScholarPubMed
Emerson, S. (1963). Slime: A plasmoid variant of Neurospora crassa. Genetics 34, 162–182.Google Scholar
Garnjobst, L. & Tatum, E. L. (1967). A survey of new morphological mutants in Neurospora. Genetics, 57, 579–603.CrossRefGoogle ScholarPubMed
Giles, N. H. (1951). Studies on the mechanism of reversion in biochemical mutants of Neurospora crassa. Cold Spring Harbor Symposia on Quantitative Biology 16, 283–313.CrossRefGoogle ScholarPubMed
Giles, N. H., Case, M. E., Partridge, C. W. H. & Ahmed, S. I. (1967). A gene cluster coding for an aggregate of five aromatic synthetic enzymes. Proceedings of the National Academy of Sciences of the United States of America 58, 1453–1460.CrossRefGoogle ScholarPubMed
Hamilton, J. G. & Calvet, J. (1964). Production of protoplast in an osmotic mutant of Neurospora crassa without added enzyme. Journal of Bacteriology 88, 1084–1086.CrossRefGoogle Scholar
Herdman, M. (1973). Mutations arising during transformations in the blue green alga Anacystis nidulans. Molecular and General Genetics 120, 369–378.CrossRefGoogle ScholarPubMed
Howe, M. M. & Bade, E. G. (1975). Molecular Biology of Bacteriophage Mu. Science 190, 624–632.CrossRefGoogle ScholarPubMed
Mays, L. L. (1969). Isolation, characterization and genetic analysis of osmotic mutants of Neurospora crassa. Genetics 63, 781–794.CrossRefGoogle ScholarPubMed
Mishra, N. C. (1971). Heterokaryosis in Neurospora sitophila. Genetics 67, 55–59.CrossRefGoogle ScholarPubMed
Mishra, N. C. (1976). Surface architecture of the different strains of Neurospora crassa as revealed by scanning electron microscopy and their relation to morphology. Journal de Microscopie et de Biologie Cellulaire 26, 151–159.Google Scholar
Mishra, N. C. (1977). Genetics and Biochemistry of morphogenesis in Neurospora. In Advance in Genetics, vol 19 (ed. Caspari, E.). New York: Academic Press (in the Press).Google Scholar
Mishra, N. C., Szabo, G. & Tatum, E. L. (1973). Nucleic acid-mediated genetic changes in Neurospora. In The role of RNA in Reproduction and Development (ed. Niu, M. C. and Segal, S.), pp. 259–268. Amsterdam: North Holland Publ. Co.Google Scholar
Mishra, N. C. and Tatum, E. L. (1973). Non-medelian inheritance of DNA induced inositol independence in Neurospora. Proceedings of the National Academy of Sciences of the United States of America 70, 3875–3879.CrossRefGoogle Scholar
Mishra, N. C. & Threlkeld, S. F. H. (1967). Variation in the expression of ragged mutants in Neurospora. Genetics 55, 113–121.CrossRefGoogle ScholarPubMed
Perkins, D. D. (1959). New markers and multiple point linkage data in Neurospora. Genetics 44, 1185–1208.CrossRefGoogle ScholarPubMed
Pittenger, T. (1964). The general incidence of pseudowild type in Neurospora crassa. Genetics 39, 326–342.CrossRefGoogle Scholar
Rand, J. (1975). The regulation of sugar transport and galactose metabolism in Neurospora crassa. Ph.D. thesis, The Rockefeller University, New York, N.Y. pp. 1–229.Google Scholar
Scarborough, G. A. (1973). Transport in Neurospora. International Review of Cytology 30, 103–122.CrossRefGoogle Scholar
Tatum, E. L., Barratt, R. W. & Cutter, V. M. (1940). Paramorphogenic effects of L-Sorbose on Neurospora and Syncephalastrum. Science 109, 509–511.CrossRefGoogle Scholar
Taylor, A. L. (1963). Bacteriophage-induced mutation in E. coli. Proceedings of the National Academy of Sciences of the United States of America 50, 1043–1051.CrossRefGoogle Scholar
Vogel, H. J. (1964). Evolution of lysine pathways. I. Ann. Naturalist 98, 435–469.CrossRefGoogle Scholar
Whitehouse, H. L. K. (1965). Towards an Understanding of the Mechanism of Heredity, 3rd edition, pp. 327–371. New York: St Martin's Publisher.Google Scholar
Yoshikawa, H. (1966). Mutations resulting from the transformation of Bacillus subtilis. Genetics 54, 1201.CrossRefGoogle ScholarPubMed