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Characterization of MR (P) strains of Drosophila melanogaster: the number of intact P elements and their genetic effect

Published online by Cambridge University Press:  14 April 2009

Jan C. J. Eeken ,
Ron J. Romeyn ,
Anja W. M. De Jong ,
George Yannopoulos  and
Albert Pastink
Jan C. J. Eeken*
Affiliation:
Department of Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratories, State University of Leiden, Wassenaarseweg 72, Leiden, The Netherlands J. A. Cohen Interuniversity ResearchInstitute for Radiopathology and Radiation Protection, Leiden, The Netherlands
Ron J. Romeyn
Affiliation:
Department of Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratories, State University of Leiden, Wassenaarseweg 72, Leiden, The Netherlands J. A. Cohen Interuniversity ResearchInstitute for Radiopathology and Radiation Protection, Leiden, The Netherlands
Anja W. M. De Jong
Affiliation:
Department of Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratories, State University of Leiden, Wassenaarseweg 72, Leiden, The Netherlands J. A. Cohen Interuniversity ResearchInstitute for Radiopathology and Radiation Protection, Leiden, The Netherlands
George Yannopoulos
Affiliation:
Department of Genetics, University of Patras, Patras, Greece
Albert Pastink
Affiliation:
Department of Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratories, State University of Leiden, Wassenaarseweg 72, Leiden, The Netherlands
*
*Corresponding author.
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To study the effect of mutagenic/carcinogenic agents on P-element transposition, the P strains used should be denned, especially with respect to the number of intact and functional P elements present. In this investigation, the relation between the number of complete P elements present in dysgenic males and P-insertion mutagenesis was studied in several MR (P) strains. The main conclusions from this investigation are: (1) Complete P elements can be present in the genome without genetic activity (even in a ‘dysgenic’ cross). As a consequence, the number of complete P elements present in particular dysgenic flies, is not necessarily an indication of their dysgenic genetic activity. (2) The MR-h12/Cy strain carries two complete P elements, one on the X chromosome without and one on the MR chromosome with genetic activity (making this strain most suitable for studies on P-transposition mechanisms).

Type
Research Article
Information
Genetics Research , Volume 58 , Issue 3 , December 1991 , pp. 211 - 223
Copyright
Copyright © Cambridge University Press 1991

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