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Characterization and mapping of a viable anaemic mutant in the mouse: a new allele, mkvan, at the microcytic anaemia locus

Published online by Cambridge University Press:  14 April 2009

Sunil Handa ,
Janet M. Ferguson ,
Margaret E. Wallace  and
Grahame Bulfield
Sunil Handa
Affiliation:
Department of Haematology, Royal Infirmary of Edinburgh, Edinburgh EH3 9YW, U.K.
Janet M. Ferguson
Affiliation:
Department of Genetics, University of Cambridge, Cambridge CB2 3EH, U.K.
Margaret E. Wallace
Affiliation:
Department of Genetics, University of Cambridge, Cambridge CB2 3EH, U.K.
Grahame Bulfield*
Affiliation:
Gene Expression Group, AFRC Institute of Animal Physiology and Genetic Research, Edinburgh Research Station, Roslin, Midlothian, EH25 9PS, U.K.
*
* Corresponding author.
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Summary

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A viable anaemic mouse mutant arose in the stock of a mouse fancier and has been characterized haematologically and genetically. Anaemic animals were less viable than normal animals (especially from 0 to 2 weeks of age) and had lower haemoglobin levels, percentage packed-cell volumes, higher red cell counts and lower mean cell volume than normal animals. Peripheral blood films showed a wide range of abnormal cells and extreme microcytosis. Linkage studies showed the mutant to be linked to the chromosome 15 markers Na Ca and bt; recombination with Ca was 1·37 ± 0·68 % for females and 10·5 ± 7·41 % for males. This position is similar to the microcytic anaemia, mk, mutant, and crosses between the viable anaemia mutation and mk/mk homozygotes showed the two to be allelic. Viable anaemia is therefore a second allele at the mk locus mkvan; new data give its position on chromosome 15.

Type
Research Article
Information
Genetics Research , Volume 51 , Issue 1 , February 1988 , pp. 41 - 45
Copyright
Copyright © Cambridge University Press 1988

References

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