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Characteristics of E. coli K12 strains carrying both an F prime and an R factor

Published online by Cambridge University Press:  14 April 2009

N. S. Willetts  and
D. J. Finnegan
N. S. Willetts
Affiliation:
M.R.C. Molecular Genetics Unit, Department of Molecular Biology, University of Edinburgh, Scotland
D. J. Finnegan
Affiliation:
M.R.C. Molecular Genetics Unit, Department of Molecular Biology, University of Edinburgh, Scotland
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The interrelationship between an F prime and an F-like or I-like R factor was studied in Escherichia coli K 12 strains carrying both. The donorability of such strains suggested that these plasmids were transferred independently by their own transfer systems. Plaque formation by several male- and female-specific phage was tested; the inhibition by Flac of several female-specific phage was not affected by an F-like R factor. Neither did an R factor affect the incompatibility between Flac and Fhis. However, the surface exclusion characteristic of Flac was lost in the presence of an F-like R factor. This is probably closely related to the simultaneous loss of donorability, the F-pilus, and the f+ antigen: all may be the result of the inhibition by an R product of the formation or activity of a single F product.

Type
Research Article
Information
Genetics Research , Volume 16 , Issue 1 , August 1970 , pp. 113 - 122
Copyright
Copyright © Cambridge University Press 1970

References

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