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Changes in genetic constitution and sterol composition during growth of nystatin-resistant heterokaryons of Neurospora crassa

Published online by Cambridge University Press:  14 April 2009

Jill E. Ogden  and
Morris Grindle
Jill E. Ogden
Affiliation:
Department of Genetics, University of Sheffield, Sheffield S10 2TN
Morris Grindle
Affiliation:
Department of Genetics, University of Sheffield, Sheffield S10 2TN
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Heterokaryons of N. crassa were synthesized from homokaryotic strains differing in sterol composition and sensitivity to the polyene antibiotic nystatin. Mycelia of the nystatin-sensitive strain erg-1+ contained ergosterol and episterol, and the nystatin-resistant mutant erg-1 contained fecosterol and lichesterol. Mycelia of heterokaryons with different proportions of erg-1+: erg-1 nuclei contained various proportions of the four sterols. Ergosterol was the principal sterol in heterokaryons with more than 5% erg-1+ nuclei.

Heterokaryons with various proportions of erg-1+:erg-1 nuclei were grown for several weeks along tubes of synthetic media. Growth rates were stable on minimal medium and nutritionally supplemented media but nuclear proportions often fluctuated. Growth rates fell sharply on nystatin-supplemented media and there were adaptive increases in proportions of mutant erg-1 nuclei which resulted in selection of nystatin-resistant homokaryotic mycelia.

Type
Research Article
Information
Genetics Research , Volume 42 , Issue 1 , August 1983 , pp. 91 - 103
Copyright
Copyright © Cambridge University Press 1983

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