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Biochemical investigation of the slow-growing non-perithecial (sgp) mutants of Aspergillus nidulans

Published online by Cambridge University Press:  14 April 2009

J. A. Houghton
J. A. Houghton
Affiliation:
Department of Genetics, The University, Liverpool, England
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Using polarography the uptake of oxygen by intact and homogenized mycelium of a wild-type strain and strains of slow-growing non-perithecial (sgp) mutants was compared. It was found that whilst the oxygen uptake of intact, wild-type mycelium increased on the addition of glucose or succinate as substrate, uptake of oxygen by mutant mycelium increased only when glucose was used as substrate and was unaffected by succinate. When, however, homogenates of mutant mycelium were used oxidation of the succinate occurred. It was concluded that the inability of the mutants to utilize succinate was due to their impaired ability to take up the compound across the hyphal wall and this was confirmed using radioactively labelled substrates. It is tentatively suggested that the abnormal growth of the sgp mutants on glucose medium and their impaired permeability to tricarboxylic acid cycle intermediates may be due to reduced availability of high energy compounds caused by a lesion in their oxidative phos-phorylation system.

Type
Research Article
Information
Genetics Research , Volume 17 , Issue 3 , June 1971 , pp. 237 - 244
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Cirillo, V. P. (1961). Sugar transport in microorganisms. Annual Review of Microbiology 15, 197–218.CrossRefGoogle Scholar
Gomari, G. (1955). In Methods in Enzymology, Vol. I, pp. 138–146 (ed. Colowick, S. P. & Kaplan, N. O.). Academic Press.CrossRefGoogle Scholar
Houghton, J. A. (1970). A new class of slow-growing non-perithecial mutants of Aspergillus nidulans. Genetical Research 16, 285–292.CrossRefGoogle ScholarPubMed
Jacobs, E. E., Jacob, M., Sanadi, D. R. & Bradley, L. B. (1956). Uncoupling of oxidative phosphorylation by cadmium ion. Journal of Biological Chemistry 223, 147–156.CrossRefGoogle Scholar
Kovac, L. & Hrusovska, E. (1968). Oxidative phosphorylation in yeast. II. An oxidative phosphorylation-deficient mutant. Biochimica et Biophysica Acta 153, 43–54.CrossRefGoogle Scholar
Kovac, L., Lachowicz, T. M. & Slonimski, P. P. (1967). Biochemical genetics of oxidative phosphorylation. Science, New York 158, 1564–1567.CrossRefGoogle ScholarPubMed
Lennox, J. E. & Tuveson, R. W. (1967). The isolation of ultraviolet sensitive mutants from Aspergillua ruguloaus. Radiation Research 31, 382–388.CrossRefGoogle Scholar
Moses, V. (1969). In Chromatographic and Electrophoretic Techniques, pp. 484–533 (Ed. Smith, I.). London: Heinemann.Google Scholar
Nagai, S., Yanagishima, N. & Nagai, H. (1961). Advances in the study of respiration-deficient (RD) mutation in yeast and other micro-organisms. Bacteriological Reviews 25, 404–426.CrossRefGoogle Scholar
Pontecorvo, G., Roper, J. A., Hemmons, L. M., Macdonald, K. D. & Bufton, A. W. J. (1953). The genetics of Aspergillus nidulans. Advances in Genetics 5, 141–238.CrossRefGoogle ScholarPubMed
Roberts, C. F. (1963). The adaptive metabolism of D-galactose in Aspergillus nidulans. Journal of General Microbiology 31, 285–295.CrossRefGoogle ScholarPubMed
Watson, K. & Smith, J. E. (1967). Oxidative phosphorylation and respiratory control in mitochondria from Aspergillus niger. Biochemical Journal 104, 332–339.CrossRefGoogle ScholarPubMed