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Assaying chromosome arrangement in embryonic interphase nuclei of Drosophila melanogaster by radiation induced interchanges

Published online by Cambridge University Press:  14 April 2009

Arthur J. Hilliker
Arthur J. Hilliker
Affiliation:
Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario, Canada N1G 2W1
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Summary

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Despite recent advances in our understanding of chromatin ultrastructure, little is known of the arrangement of chromosomes during interphase, the portion of the cell cycle associated with somatic gene transcription. An experimental procedure is described which has allowed the determination of the nature of the relative arrangement during interphase of chromosomes in a specific diploid cell type of Drosophila, the salivary gland anlage of the 10–14-h-old embryo. At this stage of development the salivary gland cells have ceased mitotic divisions. Embryos of 10–14 h in age were irradiated with 12000 rads of gamma radiation and then allowed to develop into third instar larvae. The polytene chromosomes of these larvae were examined for radiation-induced interchanges. From the distribution of observed interchanges, three major features of interphase chromosome arrangement were inferred. (1) Each euchromatic chromosomal arm occupies a specific domain within the interphase nucleus which does not appreciably overlap with those of other arms. (2) Within these chromosomal domains DNA folding is very extensive. (3) The heterochromatic regions of each chromosomal arm are sequestered from the euchromatic regions. An additional point of interest concerns the nature of the interchanges observed. No reciprocal interchanges were observed – all appeared to be partial exchanges, possibly subchromatid interchanges involving only one DNA strand from each of the two exchange sites.

Type
Research Article
Information
Genetics Research , Volume 47 , Issue 1 , February 1986 , pp. 13 - 18
Copyright
Copyright © Cambridge University Press 1986

References

Avivi, L. & Feldman, M. (1980). Arrangement of chromosomes in the interphase nucleus of plants. Human Genetics 55, 281–295.CrossRefGoogle ScholarPubMed
Berendes, H. D. & Ashburner, M. (1978). The salivary gland. In The Genetics and Biology of Drosophila, vol. 2b. pp. 453–488.Google Scholar
Bownes, M. (1975). A photographic study of development in the living embryo of Drosophila melanogaster. Journal of Embryology and Experimental Morphology 33, 789–801.Google ScholarPubMed
Bridges, C. B. (1935). Salivary chromosome maps. Journal of Heredity 26, 60–64.CrossRefGoogle Scholar
Bridges, C. B. (1938). A revised map of the salivary gland X chromosome of Drosophila melanogaster. Journal of Heredity 29, 11–13.CrossRefGoogle Scholar
Bridges, C. B. & Bridges, P. N. (1939). A new map of the second chromosome: a revised map of the right limb of the second chromosome of Drosophila melanogaster. Journal of Heredity 30, 475–476.CrossRefGoogle Scholar
Bridges, P. N. (1941 a). A revised map of the left limb of the third chromosome of Drosophila melanogaster. Journal of Heredity 32, 64–65.CrossRefGoogle Scholar
Bridges, P. N. (1941 b). A revision of the salivary gland 3R-chromosome map of Drosophila melanogaster. Journal of Heredity 32, 299–300.CrossRefGoogle Scholar
Bridges, P. N. (1942). A new map of the salivary gland 2L-chromosome of Drosophila melanogaster. Journal of Heredity 33, 403–408.CrossRefGoogle Scholar
Comings, D. E. (1980). Arrangement of chromatin in the nucleus. Human Genetics 8, 131–143.Google Scholar
Cremer, T., Cremer, C., Schneider, T., Baumann, H., Hens, L. & Kirsch-Volders, M. (1982). Analysis of chromosome positions in the interphase nucleus of Chinese hamster cells by laser UV-microirradiation experiments. Human Genetics 62, 201–209.CrossRefGoogle ScholarPubMed
Foster, G. F., Whitten, M. J., Konovalov, C., Arnold, J. T. A. & Maffi, G. (1981). Autosomal genetic maps of the Australian sheep blowfly, Lucilia cuprina dorsalis R.-D. (Diptera; Calliphoridae) and possible correlations with the linkage maps of Musca domestica L. and Drosophila melanogaster (Mg.). Genetical Research 37, 55–69.CrossRefGoogle Scholar
Fullilove, S. L. & Jacobson, A. G. (1978). Embryonic development: Descriptive. In The Genetics and Biology of Drosophila, vol. 2c, pp. 105–227. London: Academic Press.Google Scholar
Hager, H. D., Schroeder-Kurth, T. M. & Vogel, F. (1982). Positions of chromosomes in the human interphase nucleus. An analysis of nonhomologous chromatid translocations in lymphocyte cultures after Trenimon treatment and from patients with Fanconi's Anemia and Bloom's Syndrome. Human Genetics 61, 342–356.Google ScholarPubMed
Hens, L., Baumann, H., Cremer, T., Sutter, A., Cornelis, J. J. & Cremer, C. (1983). Immunocytochemical localization of regions UV-microirradiated in S phase or anaphase. Evidence for a territorial organization of chromosomes during cell cycle of cultured Chinese hamster cells. Experimental Cell Research 149, 257–269.CrossRefGoogle ScholarPubMed
Igo-Kemenes, T., Horz, W. & Zachau, H. G. (1982). Chromatin. Annual Review of Biochemistry 51, 89–121.CrossRefGoogle ScholarPubMed
Mathog, D., Hochstrasser, M., Gruenbaum, Y., Sawmweber, H., and Sedat, J. (1984). Characteristic folding pattern of polytene chromosomes in Drosophila salivary gland nuclei. Nature 308, 414–421.CrossRefGoogle ScholarPubMed
Rudkin, G. T. (1969). Nonreplicating DNA in Drosophila. Genetics (Suppl.) 61, 227–238.Google Scholar
Sturtevant, A. H. & Novitski, E. (1941). The homologies of the chromosome elements in the genus Drosophila. Genetics 26, 517–541.CrossRefGoogle ScholarPubMed
Wilson, E. B. (1925). The Cell in Development and Heredity, 3rd ed. New York: Macmillan.Google Scholar
Zorn, C., Cremer, C., Cremer, T. & Zimmer, J. (1979). Unscheduled DNA synthesis after partial UV irradiation of the cell nucleus. Distribution in interphase and metaphase. Experimental Cell Research 124, 111–119.CrossRefGoogle ScholarPubMed