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The application of tissue-culture techniques to the chromosomal analysis of Bos taurus

Published online by Cambridge University Press:  14 April 2009

R. Crossley  and
G. Clarke
R. Crossley
Affiliation:
Medical Research Council, Population Genetics Research Unit, Old Road, Headington, Oxford
G. Clarke
Affiliation:
Medical Research Council, Population Genetics Research Unit, Old Road, Headington, Oxford
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Until 1960 all the chromosome counts performed on cells of this species were done on histologically prepared specimens of testicular tissue. In January 1960, Chiarelli et al. published the results of their experiments to grow renal tissue in culture. Their specimens were obtained from male and female calves. They confirmed that 2n = 60 and described the chromosomes as being all acrocentric except the X chromosome, which was submetacentric.

The purpose of the work described below was to try to apply the peripheral-blood culture technique of Moorhead to bovine blood, and to attempt to grow bovine muscle cells in a tissue culture.

Type
Short Notes
Information
Genetics Research , Volume 3 , Issue 1 , February 1962 , pp. 167 - 168
Copyright
Copyright © Cambridge University Press 1962

References

REFERENCES

Chiarelli, B., De Carli, L. & Nuzzo, F. (1960). Analisi morphometica dei chromosomi de Bos taurus L. Caryologia, 13, No. 3.Google Scholar
Harnden, D. G. (1960). A human skin culture technique used for cytological examination. Brit. J. exp. Path. 41, 31–37.Google Scholar
Hsu, T. C. & Kellogg, D. S., Jun. (1960). Primary cultivation and continuous propogation in vitro of tissues from small biopsy specimens. J. nat. Cancer Inst. 25, 221–235.Google Scholar
Moorhead, P. S., Nowell, P. C, Mellman, W. J., Battips, D. M. & Hungerford, D. A. (1960). Chromosome preparations of leukocytes cultured from human peripheral blood. Exp. Cell Res. 20, 613–616.CrossRefGoogle ScholarPubMed