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Analysis of lines of mice selected for fat content: 3. Flux through the de novo lipid synthesis pathway

Published online by Cambridge University Press:  14 April 2009

Emmanuel A. Asante ,
William G. Hill  and
Grahame Bulfield
Emmanuel A. Asante
Affiliation:
Cellular and Molecular Biology Department, AFRC Institute of Animal Physiology and Genetics, Edinburgh Research Station, Roslin, Midlothian EH25 9PS
William G. Hill
Affiliation:
Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT*
Grahame Bulfield
Affiliation:
Cellular and Molecular Biology Department, AFRC Institute of Animal Physiology and Genetics, Edinburgh Research Station, Roslin, Midlothian EH25 9PS
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The flux through the de novo fatty acid synthesis pathway was estimated in lines of mice which differed substantially in fat content following 26 generations of selection at 10 weeks of age. Previous estimates of lipogenic enzyme activities had indicated an increase in the capacity for lipogenesis in the Fat compared to the Lean line. Therefore the in vivo flux in lipogenesis was measured in both liver and gonadal fat pad (GFP) tissues of males at 5 and 10 weeks of age, using the rat of incorporation of 3H from 3H2O and 14C from acetate and citra te into total lipids. AT both ages and in both tissues the Fat line had a higher flux, about 20% increase in the liver and up to three-fold increase (range 1·2- to 3·4-fold) in the GFP. We conclude that direct selection for fatness in mice has resulted in metabolic changes in the ratio of de novo fatty acid synthesis, and that the changes are largely detectable before 10 weeks, the age of selection.

Type
Research Article
Information
Genetics Research , Volume 58 , Issue 2 , October 1991 , pp. 123 - 127
Copyright
Copyright © Cambridge University Press 1991

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