Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-24T13:52:10.946Z Has data issue: false hasContentIssue false
  • English
  • Français

Analysis of lines of mice selected for fat content. 1. Correlated responses in the activities of NADPH-generating enzymes.

Published online by Cambridge University Press:  14 April 2009

Emmanuel A. Asante ,
William G. Hill  and
Grahame Bulfield
Emmanuel A. Asante
Affiliation:
Gene Expression Group, AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian EH25 9PS
William G. Hill
Affiliation:
Institute of Animal Genetics, University of Edinburgh, West Mains Road, Edinburgh EH9 3JN
Grahame Bulfield*
Affiliation:
Gene Expression Group, AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian EH25 9PS
*
* Corresponding author.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Estimates of the activities (Vmax) of four enzymes that generate the coenzyme NADPH, an absolute requirement for tissue fatty-acid synthesis, and of the concentration of NADP plus NADPH were made in lines of mice differing in fat content. These lines had been selected from the same base population for 20 generations, and 3 high, 3 low replicates and 1 unselected control were used. Analyses were performed on liver and gonadal fat pad (GFP) of males at 5 and 10 weeks of age. In both the liver and the GFP, measurable activities of the four enzymes: glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), isocitrate dehydrogenase (IDH) and malic enzyme (ME) expressed per mg soluble protein were, with minor exceptions, higher in the Fat (F) than in the Lean (L) lines at both ages; the highest ratio being 2–2 for ME in the GFP. The relationships between these measurable activities (Vmax) and in vivo lipogenesis are not however known. When expressed per gram tissue, the ratios for F to L in the GFP were less than 1 in most cases, presumably because of the very different adipocyte numbers and/or sizes between the lines. There were no significant differences between the lines in the concentration of NADP plus NADPH per gram tissue in liver or GFP, suggesting that F lines converted NADP to NADPH faster than L lines. It is predicted that selection on the enzyme activities would be less efficient than direct selection at changing fat content.

Type
Research Article
Information
Genetics Research , Volume 54 , Issue 2 , October 1989 , pp. 155 - 160
Copyright
Copyright © Cambridge University Press 1989

References

Ballard, F. J. & Hanson, R. W. (1967). Changes in synthesis in rat liver during development. Biochemical Journal 102, 952–958.Google Scholar
Bulfield, G. & Moore, E. A. (1974). Semi-automated assays for enzymopathies of carbohydrate metabolism in liver and erythrocytes, using a reaction rate analyser. Clinica Chimica Acta 53, 265–271.CrossRefGoogle ScholarPubMed
Falconer, D. S. (1983). Introduction to Quantitative Genetics, 2nd edn. pp. 340. Longman.Google Scholar
Gandemer, G., Pascal, G. & Durand, G. (1985). Comparative changes in the in vivo fatty acid synthesis in liver and adipose tissues during the post-weaning growth of male rats. Comparative Biochemical Physiology 82B, 581–586.Google Scholar
Hastings, I. M. & Hill, W. G. (1989). A note on the effect of different selection criteria on carcass composition in mice. Animal Production 48, 229–233.Google Scholar
Hill, W. G. (1985). Detection and genetic assessment of physiological criteria of merit within breeds. In Genetics of Reproduction in Sheep (ed. Land, R. B. and Robinson, D. W.), pp. 319–331. Butterworths.Google Scholar
Hudson, G. F. S. & Kennedy, B. W. (1985). Genetic trend of growth rate and backfat thickness of swine in Ontario. Journal of Animal Science 61(1), 92–97.Google Scholar
Kather, H. & Brand, K. (1985). Origin of hydrogen required for fatty acid synthesis in isolated rat adipocytes. Archives of Biochemistry and Biophysics 170, 417–426.Google Scholar
Langdon, R. G. (1957). The biosynthesis of fatty acids in rat liver. Journal Biological Chemistry 226, 615–629.Google Scholar
Leclercq, B., Blum, J. C. & Boyer, J. P. (1980). Selecting broilers for low or high abdominal fat: initial observations. British Poultry Science 21, 107–113.Google Scholar
Madvig, P. & Abraham, S. (1980). Relationship of Malic enzyme activity to fatty acid synthesis and the pathways of glucose catabolism in developing rat liver. Journal of Nutrition 110, 90–99.Google Scholar
Muller, E. (1985). Genetic regulation of fat metabolism in pigs. Paper presented at the 36th Annual Meeting of the Study Commission EAAP, Kallithea, Halkidiki, Greece, 30 September-3 October, 1985.Google Scholar
Muller, E. (1986). Physiological and biochemical indicators of growth and composition. In Exploiting New Technologies in Animal Breeding, Genetic Developments (ed. Smith, C., , J. W., King, B. and McKay, J. C.), pp. 132–139. Oxford Science Publications.Google Scholar
Nisselbaum, J. S. & Green, S. (1969). A simple ultramicro method for determination of pyridine nucleotides in tissues. Analytical Biochemistry 27, 212–217.Google Scholar
Sharp, G. L., Hill, W. G. & Robertson, A. (1984). Effects of selection on growth, body composition and food intake in mice 1. Responses in selected traits. Genetical Research 43, 75–92.CrossRefGoogle ScholarPubMed