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An extensive behavioural and genetic analysis of the Pawn mutants in Paramecium aurelia

Published online by Cambridge University Press:  14 April 2009

Sheng-Yung Chang ,
Judith Van Houten ,
Laura J. Robles ,
Simmy S. Lui  and
Ching Kung
Sheng-Yung Chang
Affiliation:
Department of Biological Sciences, University of California, Santa Barbara, California 93106, U.S.A.
Judith Van Houten
Affiliation:
Department of Biological Sciences, University of California, Santa Barbara, California 93106, U.S.A.
Laura J. Robles
Affiliation:
Department of Biological Sciences, University of California, Santa Barbara, California 93106, U.S.A.
Simmy S. Lui
Affiliation:
Department of Biological Sciences, University of California, Santa Barbara, California 93106, U.S.A.
Ching Kung
Affiliation:
Department of Biological Sciences, University of California, Santa Barbara, California 93106, U.S.A.
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Pawns are behavioural mutants which show impairment in membrane excitability and are, therefore, devoid of normal avoiding reactions. We obtained 103 lines of Pawns through mutagenesis and screening. Among those that yielded to breeding studies, 59 lines belonged to the pwA complementation group and 38 lines belonged to the pwB group. No other genic loci were found in this extensive analysis. Mutants of the pwA locus showed various degrees of phenotypic leakiness. Based on the pheno- and genotypic differences, we estimated that at least 45 independent mutational events were represented in these 103 Pawn lines.

Type
Research Article
Information
Genetics Research , Volume 23 , Issue 2 , April 1974 , pp. 165 - 173
Copyright
Copyright © Cambridge University Press 1974

References

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