Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-24T06:46:29.677Z Has data issue: false hasContentIssue false
  • English
  • Français

Allelic variation at the Gli-A1m, Gli-A2m and Glu-A1m loci and breadmaking quality in diploid wheat Triticum monococcum

Published online by Cambridge University Press:  14 April 2009

C. Saponaro ,
N. E. Pogna ,
R. Castagna ,
M. Pasquini ,
P. Cacciatori  and
R. Redaelli
C. Saponaro
Affiliation:
Istituto Sperimentale per la Cerealicoltura, Applied Genetics Section, via Cassia 176, 00191 Rome
N. E. Pogna*
Affiliation:
Istituto Sperimentale per la Cerealicoltura, Applied Genetics Section, via Cassia 176, 00191 Rome
R. Castagna
Affiliation:
Section of S. Angelo Lodigiano, Via Molino 3, 20079 S. Angelo Lodigiano, Italy
M. Pasquini
Affiliation:
Istituto Sperimentale per la Cerealicoltura, Applied Genetics Section, via Cassia 176, 00191 Rome
P. Cacciatori
Affiliation:
Istituto Sperimentale per la Cerealicoltura, Applied Genetics Section, via Cassia 176, 00191 Rome
R. Redaelli
Affiliation:
Section of S. Angelo Lodigiano, Via Molino 3, 20079 S. Angelo Lodigiano, Italy
*
* Corresponding author.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Fifty-six accessions of Triticum monococcum and one accession each of T. beoticum and T. sinskajae were analysed for their storage protein compositions and breadmaking quality as determined by the SDS-sedimentation test. In total 30 different alleles at the Glu-A1m locus coding for high-molecular-weight glutenin subunits (HMW-GS), 25 alleles at the Gli-A1m locus coding for ω- and γ-gliadins and 45 alleles at the Gli-A2m locus controlling the synthesis of α/β-gliadins were detected. Most accessions contained one x-type and one y-type HMW-GS and two genotypes were null for both types of subunits. Two polypeptides within the mobility range of HMW-GS in SDS-PAGE were shown to be ω-type gliadins encoded by genes on the short arm of chromosome 1 A. T. sinskajae and several ‘monococcum’ accessions were shown to share the same alleles at Gli-A1m, Gli-A2m and Glu-A1m, confirming sinskajae as a subspecies of T. monococcum. The SDS-sedimentation volumes of most accessions were very low (11–35 ml), a few accessions showing mean sedimentation volumes as high as 90–93 ml. Through the comparison between biotypes occurring in some accessions of ‘monococcum’, good bread-making quality was found to be associated with the presence of alleles y, c and i at the Gli-A1m locus. All accessions were resistant to leaf rust and rich in protein (≥ 16·5%), and most of them showed resistance to powdery mildew.

Type
Research Article
Information
Genetics Research , Volume 66 , Issue 2 , October 1995 , pp. 127 - 137
Copyright
Copyright © Cambridge University Press 1995

References

Blanco, A., Zacheo, S., Antonacci, M., Tribuzio, G. & Bufano, A. G. (1994). Frumenti diploidi selvatici e miglioramento della qualità del frumento duro. In IV Giornate Internazionali sul Grano Duro (ed. Fonzo, N. Di and Ronga, G.), pp. 101–115, Camera di Commercio, Foggia, Italy.Google Scholar
Borojevic, S. (1956). A note above the “New data for recent cultivation of T. monococcum and T. dicoccum in Yugoslavia”. Wheat Information Service 4, 1.Google Scholar
Castagna, R., Maga, G., Perenzin, M., Heun, M. & Salamini, F. (1994). RFLP based genetic relationship of einkorn wheats. Theoretical and Applied Genetics (in the Press).CrossRefGoogle Scholar
Dachkevitch, T., Redaelli, R., Biancardi, A. M., Metakovsky, E. V. & Pogna, N. E. (1993). Genetics of gliadins coded by the group 1 chromosomes in the high-quality bread wheat cultivar Neepawa. Theoretical and Applied Genetics 86, 389–399.CrossRefGoogle Scholar
D'Egidio, M. G., Nardi, S. & Vallega, V. (1993). Grain, flour and dough characteristics of selected strain of diploid wheat, Triticum monococcum L. Cereal Chemistry 70, 298–303.Google Scholar
Fedak, G. (1985). Alien species as sources of physiological traits for wheat improvement. Euphytica 34, 673–680.CrossRefGoogle Scholar
Galili, S., Felsenburg, T., Levy, A. A., Altschuler, Y. & Feldman, M. (1988). Inactivity of high-molecular-weight glutenin genes in wild diploid and tetraploid wheats. Proceedings of the 7th International Wheat Genetics Symposium (ed. Miller, T. E. and Koebner, R. M. D.), pp. 81–86, IPSR, Cambridge, UK.Google Scholar
Gorham, S., Bristol, A., Young, E. M. & Wyn Jones, R. G. (1991). The presence of the enhanced K/Na discrimination trait in diploid Triticum species. Theoretical and Applied Genetics 82, 729–736.CrossRefGoogle ScholarPubMed
Gupta, R. B. & Shepherd, K. W. (1988). Low-molecular-weight glutenin subunits in wheat: their variation, inheritance and association with bread-making quality. Proceedings of the 7th International Wheat Genetics Symposium (ed. Miller, T. E. and Koebner, R. M. D.), pp. 943–949. IPSR, Cambridge, UK.Google Scholar
Gupta, R. B., Bekes, F. & Wrigley, C. W. (1991). Prediction of physical dough properties from glutenin subunit composition in bread wheat: correlation studies. Cereal Chemistry 68, 328–333.Google Scholar
Gupta, R. B., Peul, S. G., Cornish, G. B., Palmer, G. A., Bekes, F. & Ratjen, A. J. (1994). Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1 of common wheat. 1. Its additive and interaction effects on dough properties. Journal of Cereal Science 19, 9–18.CrossRefGoogle Scholar
Guzy, M. R., Ehdaie, B. & Waines., J. G. (1989). Yield and its components in diploid, tetraploid and hexaploid wheats in diverse environments. Annals of Botany 64, 635–642.Google Scholar
Halford, N. G., Field, J. M., Blair, H., Urwin, P., Moore, K., Robert, L., Thompson, R., Flavell, R. B., Tatham, A. S. & Shewry, P. R. (1992). Analysis of HMW glutenin subunits encoded by chromosome 1 A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theoretical and Applied Genetics 83, 373–378.CrossRefGoogle Scholar
Johnson, B. L. & Waines, J. G. (1977). Use of wild wheat resources. California Agriculture 31, 8–9.Google Scholar
Lawrence, J. M., Day, K. M., Huey, E. & Lee, B. (1958). Lysine content of wheat varieties, species and related genera. Cereal Chemistry 35, 169–179.Google Scholar
Margiotta, B., Urbano, M., Colaprico, G., Johansson, E., Buonocore, F., D'Ovidio, R. & Lafiandra, D. (1995). Bread wheat lines with both x- and y-type subunits at the Glu-Al locus Proceedings of the Workshop ‘Wheat kernel proteins. Molecular and functional aspects’, S. Martino al Cimino, 28–30 Sept. 1994, pp. 135–138, Università della Tuscia, Viterbo, Italy.Google Scholar
Metakovsky, E. V. (1991). Gliadin allele identification in common wheat. 2. Catalogue of gliadin alleles in common wheat. Journal of Genetics & Breeding 45, 325–344.Google Scholar
Metakovsky, E. V. & Baboev, S. K. (1992 a). Polymorphism and inheritance of gliadin polypeptides in T. monococcum. Theoretical and Applied Genetics 84, 971–978.Google Scholar
Metakovsky, E. V. & Baboev, S. K. (1992 b). Polymorphism of gliadin and unusual gliadin alleles in Triticum beoticum. Genome 35, 1007–1012.Google Scholar
Pasquini, M., Corazza, L. & Perrino, P. (1989). Evaluation of Triticum monococcum and Triticum dicoccum accessions for resistance to rusts and powdery mildew. Phytopathologia Mediterranea 28, 185–188.Google Scholar
Payne, P. I. (1987). Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Annual Review of Plant Physiology 38, 141–153.Google Scholar
Payne, P. I., Corfield, K. G. & Blackman, J. A. (1979). Identification of a high-molecular-weight subunit of glutenin whose presence correlates with breadmaking quality in wheats of related pedigree. Theoretical and Applied Genetics 55, 153–159.CrossRefGoogle ScholarPubMed
Payne, P. I., Harris, P. A., Law, C. N., Holt, L. M. & Blackman, J. A. (1980). The high-molecular-weight subunits of glutenin: Structure, genetics and relationships to bread-making quality. Annals of Technology and Agriculture 29, 309–320.Google Scholar
Payne, P. I., Holt, L. M. & Law, C. N. (1981). Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. 1. Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theoretical and Applied Genetics 60, 229–236.Google Scholar
Payne, P. I., Jackson, F. A., Holt, L. M. & Law, C. N. (1984). Genetic linkage between storage protein genes on each of the short arms of chromosomes 1 A and 1 B in wheat. Theoretical and Applied Genetics 67, 235–243.Google Scholar
Payne, P. I., Seekings, J. A., Worland, A. J., Jarvis, M. C. & Holt, L. M. (1987). Allelic variation of glutenin subunits and gliadins and its effect on breadmaking quality in wheat: Analysis of F5 progeny from Chinese Spring × Chinese Spring (Hope 1A). Journal of Cereal Science 6, 103–118.Google Scholar
Pogna, N. E., Autran, J.-C., Mellini, F., Lafiandra, D. & Feillet, P. (1990). Chromosome 1 B-encoded gliadins and glutenin subunits in durum wheat: genetics and relationship to gluten strength. Journal of Cereal Science 11, 15–34.Google Scholar
Sharma, H. C. & Waines, J. G. (1981). The relationships between male and female fertility and among taxa in diploid wheats. American Journal of Botany 68, 449–451.Google Scholar
Sharma, H. C., Waines, J. G. & Foster, K. W. (1981). Variability in primitive and wild wheats for useful genetic characters. Crop Science 21, 555–559.Google Scholar
Singh, N. K. & Shepherd, K. W. (1988). Linkage mapping of genes controlling endosperm storage proteins in wheat. 1. Genes on the short arms of group 1 chromosomes. Theoretical and Applied Genetics 79, 628–641.Google Scholar
Singh, N. K., Shepherd, K. W. & Cornish, G. B. (1991). A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. Journal of Cereal Science 14, 203–208.Google Scholar
The, T. T. (1975). Variability and inheritance studies in Triticum monococcum for reaction to Puccinia graminis sp. tritici and P. recondita. Zeitscrft für Pflanzenzuechtung 76, 287–298.Google Scholar
Vallega, V. (1992). Agronomical performance and breeding value of selected strains of diploid wheat Triticum monococcum. Euphytica 61, 13–23.Google Scholar
Waines, J. G. (1983). Genetic resources in diploid wheat: The case for diploid commercial wheat. Proceedings of the 6th International Wheat Genetics Symposium, pp. 115–122.Google Scholar
Waines, J. G. & Payne, P. I. (1987). Electrophoretic analysis of the high-molecular-weight glutenin subunits of Triticum monococcum, T. urartu, and the A-genome of bread wheat (T. aestivum). Theoretical and Applied Genetics 74, 71–76.Google Scholar
Waines, J. G., Ehdaie, D. & Barnhart, D. (1987). Variability in Triticum and Aegilops species for seed characteristics. Genome 29, 41–46.Google Scholar