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Allele-specific expression of the PSP gene in the mouse sublingual glands

Published online by Cambridge University Press:  01 June 1998

JESPER LAURSEN ,
H. JAKOB LARSEN ,
THOMAS R. MIKKELSEN  and
J. PETER HJORTH
JESPER LAURSEN
Affiliation:
Department of Molecular and Structural Biology, Århus University, C.F. Møllers Allé, Bldg. 130, DK-8000 Århus, Denmark
H. JAKOB LARSEN
Affiliation:
Department of Molecular and Structural Biology, Århus University, C.F. Møllers Allé, Bldg. 130, DK-8000 Århus, Denmark Present address: Laboratory of Biochemistry B, Department of Biochemistry and Genetics, The Panum Institute, Blegdamsvej 3c, DK-2200 Copenhagen N, Denmark.
THOMAS R. MIKKELSEN
Affiliation:
Department of Molecular and Structural Biology, Århus University, C.F. Møllers Allé, Bldg. 130, DK-8000 Århus, Denmark Present address: Roskilde Katedralskole, Hobæksvej 59, DK-4000 Roskilde, Denmark.
J. PETER HJORTH
Affiliation:
Department of Molecular and Structural Biology, Århus University, C.F. Møllers Allé, Bldg. 130, DK-8000 Århus, Denmark

Abstract

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There are two known alleles of the mouse parotid secretory protein (PSP) gene: Pspa and Pspb. Pspa is carried by DBA/2J mice and Pspb is carried by C57BL/6J mice. Eighty-eight mice derived from a F1(C57BL/6J×DBA/2J) to DBA/2J backcross were analysed for PSP mRNA expression in the sublingual glands. Expression was found in heterozygous mice only. This indicates that only Pspb is expressed in this tissue. Furthermore, it maps the allele-specific sublingual gland determinant within 3·4 cM of Psp. Previous analysis of Pspb identified an enhancer-like region in position −4·6 to −3·1 kb that was necessary for transgene expression in the sublingual glands. Here it is shown that the corresponding region in Pspa enhances transgene expression in the sublingual glands as efficiently. The implications for regulation of PSP mRNA expression in the sublingual glands are discussed.

Type
Research Article
Information
Genetics Research , Volume 71 , Issue 3 , June 1998 , pp. 187 - 191
Copyright
© 1998 Cambridge University Press