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Acrosin activity in spermatozoa from sterile t6/tw32 and fertile control mice

Published online by Cambridge University Press:  14 April 2009

Ram Devi Mittal ,
Geraldine Wileman  and
Nina Hillman
Ram Devi Mittal
Affiliation:
Department of Biology, Temple University, Philadelphia, PA 19122
Geraldine Wileman
Affiliation:
Department of Biology, Temple University, Philadelphia, PA 19122
Nina Hillman*
Affiliation:
Department of Biology, Temple University, Philadelphia, PA 19122
*
*Corresponding author.
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Summary

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Spermatozoa from sterile t6/tw32 and control fertile + / +, T/tw32, T/t6 mice were compared for their abilities to hydrolyse protein matrices and for their levels of acrosin activity. The data show that the immature and mature gametes from both the experimental and control males hydrolyse protein matrices. The quantitative acrosin assays show, however, that the mature gametes from the intercomplement males have significantly less total acrosin activity than any of the control groups of gametes. These findings suggest that this reduced acrosin activity is an additional phenotypic expression of the intercomplement genotype which results in male sterility.

Type
Research Article
Information
Genetics Research , Volume 54 , Issue 2 , October 1989 , pp. 143 - 148
Copyright
Copyright © Cambridge University Press 1989

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