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Acridine treatment of F+ and Hfr strains of Escherichia coli K12 carrying a neomycin-kanamycin resistance determinant

Published online by Cambridge University Press:  14 April 2009

June N. Grindley ,
N. D. F. Grindley  and
E. S. Anderson
June N. Grindley
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9
N. D. F. Grindley
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9
E. S. Anderson
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9
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Summary

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The F factor and the K resistance determinant form an R factor (KF) in E. coli K12. In this R factor the two plasmids are independent of each other and occupy different attachment sites in the host cell.

Treatment of an F+(K) strain with acriflavine and acridine orange yielded the following classes: F+K−; F−K+; and F−K−. The K determinant was thus curable independently of F, which supports the hypothesis of independence of the two plasmids. In Hfr(K) strains, K remains curable with acridines while F is immune. Thus, K remains independent of F in both the F+ and Hfr states.

The use of higher concentrations of acridine orange than those needed for cure of K in the F+(K) strain resulted in no elimination of K, although the F factor was absent from all surviving lines tested. It is suggested that this is caused by equal inhibition of replication of both the chromosome and K, and greater inhibition of that of F.

Type
Research Article
Information
Genetics Research , Volume 15 , Issue 3 , June 1970 , pp. 327 - 334
Copyright
Copyright © Cambridge University Press 1970

References

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