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Aberrant segregation patterns and gene mappability in Ascobolus immersus

Published online by Cambridge University Press:  14 April 2009

G. Leblon ,
V. Haedens ,
A. Kalogeropoulos ,
N. Paquette  and
J.-L. Rossignol
G. Leblon
Affiliation:
Université Paris-Sud, Laboratoire de Génétique, Bât. 400, 91405 ORSAY-CEDEX, France
V. Haedens
Affiliation:
Université Paris-Sud, Laboratoire de Génétique, Bât. 400, 91405 ORSAY-CEDEX, France
A. Kalogeropoulos
Affiliation:
Université Paris-Sud, Laboratoire de Génétique, Bât. 400, 91405 ORSAY-CEDEX, France
N. Paquette
Affiliation:
Université Paris-Sud, Laboratoire de Génétique, Bât. 400, 91405 ORSAY-CEDEX, France
J.-L. Rossignol
Affiliation:
Université Paris-Sud, Laboratoire de Génétique, Bât. 400, 91405 ORSAY-CEDEX, France
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Crosses between various types of mutant giving specific patterns of aberrant segregation were performed in the b2 spore colour locus of Ascobolus immersus. The map of 41 mutations showing various patterns of aberrant segregation was established. The frequency of wild-type recombinants and the map additivity, map expansion and map contraction characteristics were shown to be strongly dependent upon the pattern of aberrant segregation of the mutations used. Mutations giving no postmeiotic segregation and an excess of conversion to wild type over conversion to mutant exhibit map expansion in small intervals and a strong map contraction in large intervals. Mutations giving postmeiotic segregations also exhibit map contraction in large intervals. Mutations giving no postmeiotic segregations and an excess of conversion to mutant over conversion to wild type show map additivity and thus provide a simple way for devising gene maps. The relationship between the mapping properties and the pattern of aberrant segregations is accounted for when considering parameters of gene conversion: frequency and distribution of hybrid DNA, frequency and direction of mismatch correction.

Type
Research Article
Information
Genetics Research , Volume 39 , Issue 2 , April 1982 , pp. 121 - 138
Copyright
Copyright © Cambridge University Press 1982

References

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