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5-azacytidine-induced decrease in the frequency of Barr body in human fibroblasts

Published online by Cambridge University Press:  14 April 2009

Asit B. Mukherjee ,
Deborah C. Luckett  and
Rene J. Herrera
Asit B. Mukherjee
Affiliation:
Department of Biological Sciences, Fordham University, Bronx, N.Y. 10458, U.S.A.
Deborah C. Luckett
Affiliation:
Department of Biological Sciences, Fordham University, Bronx, N.Y. 10458, U.S.A.
Rene J. Herrera
Affiliation:
Department of Biology and Biotechnology, Worcester Polytechnique Institute, Worcester, MA 01609, U.S.A.
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5-azacytidine-treated human fibroblasts exhibit a significant decrease in the frequencies of Barr body+ cells as compared to nontreated cultures. This presumably indicates that 5-azacytidine can induce a change in the degree of condensation of the Barr body. It is suggested that the state of chromatin condensation of the Barr body may be related to the reactivation process by 5-azacytidine of gene loci in the inactive X.

Type
Research Article
Information
Genetics Research , Volume 47 , Issue 3 , June 1986 , pp. 199 - 203
Copyright
Copyright © Cambridge University Press 1986

References

Hors-Cayla, M. C., Heuertz, S. & Frezal, J. (1983). Coreactivation of four inactive X genes in a hamster X human hybrid and persistence of late replication of reactivated X chromosome. Somatic Cell Genetics 9, 645–657.Google Scholar
Klinger, H. P., Davis, J., Goldhuber, P. & Ditta, T. (1968). Factors influencing mammalian X chromosome condensation and sex chromatin formation. I. The effect of in vitro cell density on sex chromatin frequency. Cytogenetics 7, 39–57.Google Scholar
Kondra, P. M. & Ray, M. (1978). Acridine orange fluorescence of X chromatin in cultured human fibroblasts. Cytogenetics Cell Genetics 21, 170–172.Google Scholar
Lester, S. C., Korn, N. J. & De Mars, R. (1982). Derepression of genes on the human inactive X chromosome: evidence for differences in locus-specific rates of derepression and rates of transfer of active and inctive genes after DNA-mediated transformation. Somatic Cell Genetics 8, 265–284.Google Scholar
Migeon, B. R., Schmidt, M., Axelman, J., Ruta, C. & Evans, R. (1985). Complete reversal of X inactivation in chorionic villi hybrids. American Journal of Human Genetics 37, A167 (Abstract).Google Scholar
Mohandas, T., Sparkes, R. S. & Shapiro, L. J. (1981). Reactivation of an inactive human X chromosome: evidence for X inactivation by DNA methylation. Science 2A, 393–396.Google Scholar
Mukherjee, A. B. & Czirbik, R. J. (1984). Relationship between X- and Y-chromatin body frequency, cell cycle and cell density in human fibroblasts in vitro. American Journal of Human Genetics 36, 106S (Abstract).Google Scholar
Mukherjee, A. B., Moser, G. C. & Nitowsky, H. M. (1972). Fluorescence of X and Y chromatin in human interphase cells. Cytogenetics 11, 216–227.Google Scholar
Mukherjee, A. B. & San Sebastian, J. R. (1978). Differential frequency of human X and Y chromatin as related to cell density in vitro. Cytogenetics Cell Genetics 21, 139–144.Google Scholar
Schaffer, D. A. & Priest, J. H. (1984). Reversal of DNA methylation with 5-azacytidine alters chromosome replication patterns in human lymphocyte and fibroblast cultures. American Journal of Human Genetics 36, 534–545.Google Scholar
Schmid, M., Haaf, T. & Grunert, D. (1984). 5-azacytidine-induced undercondensations in human chromosomes. Human Genetics 67, 257–263.Google Scholar
Schmidt, M., Wolf, S. F. & Migeon, B. R. (1985). Evidence for a relationship between DNA methylation and DNA replication from studies of the 5-azacytidine-reactivated allocyclic X chromosome. Experimental Cell Research 158, 301–310.Google Scholar
Viegas-Pequignot, E. & Dutrillaux, B. (1976). Segmentation of human chromosome induced by 5-ACR (5-azacytidine). Human Genetics 34, 247–254.Google Scholar
Viegas-Pequignot, E. & Dutrillaux, B. (1981). Detection of G-C rich heterochromatin by 5-azacytidine in mammals. Human Genetics 57, 124–137.Google Scholar
Wolf, S. F. & Migeon, B. R. (1982). Studies of X chromosome DNA methylation in normal human cells. Nature 295, 667–271.Google Scholar
Worsham, M. J., Van Dyke, D. L. & Weiss, L. (1985). Cytology of the reactivated human X chromosome in the human-mouse hybrid cell lines. American Journal of Human Genetics 37, A236 (Abstract).Google Scholar