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Zygotic embryo rescue in bananas

Published online by Cambridge University Press:  13 March 2008

Frédéric Bakry
Frédéric Bakry*
Affiliation:
CIRAD-Bios, UPR Multiplication végétative, avenue Agropolis, TA A-75 / 02, 34398 Montpellier Cedex 5, France
*
[email protected]
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Abstract

Introduction. This protocol describes a method for obtaining in vitro germination of zygotic embryos from open-pollinated wild seedy bananas and controlled hybridizations. The principle, key advantages, starting plant material, time required and expected results are presented. Materials and methods. This part describes the required laboratory materials, medium preparation, embryo extraction and culture. Results. Embryos typically begin germination in the dark about 15 days after inoculation on germinating medium. After 30––40 days of culture, plantlets are subcultured on a plant growth medium and placed in the light to achieve plant growth and rooting. The ratios of zygotic embryos obtained vary with the types of crosses achieved between Musa sp. genotypes.

Keywords

France (Guadeloupe)Musa sp.methodsin vitro cultureseedsembryo culturevitroplantsFrance (Guadeloupe)Musa sp.méthodeculture in vitrograineculture d'embryonvitroplant
Type
Research Article
Information
Fruits , Volume 63 , Issue 2 , March 2008 , pp. 111 - 115
Copyright
© CIRAD, EDP Sciences, 2008

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References

Bakry, F., Horry, J.-P., Tetraploid hybrids from interploid 3x / 2x crosses in cooking bananas, Fruits 47 (6) (1992) 641–647.
Bakry F., Carreel F., Caruana M.-L., Côte F.X., Jenny C., Tezenas du Montcel H., Banana, in: Charrier A., Jacquot, M., Hamon S., Nicolas D. (Eds), Tropical plant breeding, CIRAD and SPI., Plymouth, UK, 2001, pp. 1–29.
Darjo P., Bakry F., Conservation et germination des graines de bananiers (Musa sp.), Fruits 45 (2) (1990), 103–113.
Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue culture, Physiol. Plant. 15 (1962) 473–497. CrossRef