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Weaning (acclimatization) of in vitro-produced banana plants

Published online by Cambridge University Press:  23 October 2009

John Charles Robinson  and
Victor Galán Sáuco
John Charles Robinson
Affiliation:
Du Roi Lab., P.O. Box 1147, Letsitele 0885, South Africa
Victor Galán Sáuco
Affiliation:
Inst. Canario Investig. Agrar. (ICIA), Apartado Correos 60, 38208 La Laguna, Tenerife, Canary Islands, Spain
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Abstract

Introduction. The protocol describes a method for promoting the acclimatization and protection of rooted in vitro banana plants between rooting in the laboratory and their establishment in a nursery. The principle, key advantages, starting plant material and time required are presented. Materials and methods. This part describes the weaning house installation, the weaning medium used to transplant the vitroplants, the two steps for acclimatizing the plantlets: receipt of rooted laboratory plants in flasks and planting, and factors affecting the growth of the plantlets in weaning conditions (light, misting system, temperature, humidity, wind, pest control and nutrition). Possible problems for troubleshooting are listed. Results. Under normal conditions, new roots appear 4–5 days after the start of weaning, and new leaves start to grow within 8–10 days. At the end of the weaning phase, young plants are healthy, well developed and they can be established in a nursery.

Keywords

South AfricaSpainMusa sp.methodsvitroplantsweaningAfrique du SudEspagneMusa sp.méthodevitroplantsevrage
Type
Research Article
Information
Fruits , Volume 64 , Issue 5 , September 2009 , pp. 325 - 332
Copyright
© CIRAD, EDP Sciences, 2009

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