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Postharvest calcium chloride treatments do not help to increase shelf-life of bananas

Published online by Cambridge University Press:  15 April 2002

Antenette Niranjala Perera  and
Anjani Manjulika Karunaratne
Antenette Niranjala Perera
Affiliation:
Department of Botany, Faculty of Science, University of Peradeniya, Sri Lanka
Anjani Manjulika Karunaratne
Affiliation:
Department of Botany, Faculty of Science, University of Peradeniya, Sri Lanka
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Abstract

Introduction. Calcium chloride (CaCl2) treatment has been shown to increase the shelf-life of fruits, mainly through making cell walls less accessible to pathogens and softening enzymes. Materials and methods. bananas of four cultivars [`Ambon' (AAA), `Embul' (AAB), `Kolikuttu' (AAB) and `Seenikehel' (ABB)] were dipped in or pressure infiltrated with 4% CaCl2. To determine the effect of exogenous ethylene on treated fruits, they were ripened with exogenous ethylene. Ca2+ in cell wall fractions were monitored by atomic absorption spectrophotometry. Cup plate assays were performed to determine pectinase activity. Results and discussion. Pressure infiltration accelerated ripening and disease, and reduced firmness (P < 0.05). However, when exposed to ethylene, CaCl2 pressure-infiltrated bananas were insignificantly firmer than distilled water-infiltrated and ethylene-ripened bananas, showing a significant interaction (P < 0.05) between infiltration treatments and ethylene ripening. There was no consistent increase in covalently bound pectin of cell walls as seen in fruits that respond positively to CaCl2. Firmness reduction and ripening acceleration by Ca2+ treatment cannot be explained if polygalacturonase (PG) (known to be inhibited by Ca2+) was the dominant pectinase. Enzyme assays gave evidence of PG activity. When ammonium oxalate (known to bind Ca2+) was eliminated from the test medium, pectinase activity increased with increasing pH (pH 5 to 9). The presence of a pectinase enzyme which exhibits activity in the presence of Ca2+ is apparent. Conclusion. Ca2+ does not appear to influence cell wall structure of bananas but appears to influence ripening physiology.

Keywords

Sri LankaMusa (fruits)storagecalcium chloridepostharvest physiologypostharvest decaypolygalacturonaseSri LankaMusa (fruit)stockagechlorure de calciumphysiologie après récoltemaladie post récoltepolygalacturonase
Type
Research Article
Information
Fruits , Volume 57 , Issue 2 , March 2002 , pp. 87 - 94
Copyright
© CIRAD, EDP Sciences

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References

Poovaiah, B.W., Glenn, G.M., Reddy, A.S.N., Calcium and fruit softening, Physiol. Biochem. Hortic. Rev. 10 (1988) 107-152.
Stow, J., Effect of calcium ions on apple fruit softening during storage and ripening, Postharvest Biol. Tec. 3 (1993) 1-9. CrossRef
Wills, R.B.H., Tirmazi, S.I., Effect of calcium and other minerals on ripening of tomatoes, Aust. J. Plant Physiol. 6 (1979) 221-227. CrossRef
Tirmazi, S.I.H., Wills, R.B.H., Retardation of ripening of mangoes by postharvest application of calcium, Trop. Agr. 58 (1981) 137-141.
Wills, R.B.H., Tirmazi, S.I., Inhibition of ripening of avocados with calcium, Sci. Hortic. Amsterdam 16 (1982) 323-330. CrossRef
Perera O.D.A.N., A study of the physicochemical characteristics of banana and response to postharvest acid and calcium treatment, Univ. Peradeniya, M. Phil. thesis, Sri Lanka, 2000.
Perera, O.D.A.N., Karunaratne, A.M., Response of bananas to postharvest acid treatments, J. Horti. Sci. Biotech. 76 (1) (2001) 70-76. CrossRef
Sokal R.R., Rohlf F.J., Biometry, the principles and practice of statistics in biological research, 3rd, Ed. W.H. Freeman and Co., New-York, USA, 1995.
Siddiqui, S., Bangerth, F., Effect of pre-harvest application of calcium on flesh firmness and cell wall composition of apples-influence of fruit size, J. Hortic. Sci. 70 (2) (1995) 263-269. CrossRef
Dharmasiri M.A.N., A study of papaya anthracnose caused by Colletotrichum gloeosporioides (penz) Sacc. and C. Capsici (Syd) with special reference to its latent phase, Univ. Peradeniya, thesis, Sri Lanka, 1998.
Dingle, J., Reid, W.W., Solomons, G.L., The enzymic degradation of pectin and other polysaccharides. 11. Application of the "Cup Plate'' assay to the estimation of enzymes from fungal pathogens, J. Sci. Food Agr. 4 (1953) 149-155. CrossRef
Perera, O.D.A.N., Basnayake, B.M.K.M.K., Karunaratne, A.M., Physicochemical characteristics, popularity and susceptibility to anthracnose of some local banana cultivars, J. Nat. Sci. Found. Sri Lanka 27 (2) (1999) 119-130. CrossRef
Wills, R.B.H., Tirmazi, S.I., Scott, K.J., Effect of postharvest application of calcium on ripening rates of pears and bananas, J. Hortic. Sci. 57 (4) (1982) 431-435. CrossRef
Huddar, A.G., Chandramonti, H.D., Nachegowda, V., Effect of postharvest application of calcium on ripening of bananas cv. Robusta, Haryana J. Hortic. Sci. 20 (1991) 60-64.
Tanaka, K., Yokota, M., Takno, Y.I., Asakura, T., Miyairi, K., Okuno, T., Role of calcium in fruit involved in ethylene production, Actua Hortic. 197 (1998) 227-236.
Vendrell, M., McGlasson, W.B.. Inhibition of ethylene production in banana fruit tissue by ethylene treatment, Aust. J. Biol. Sci. 24 (1971) 885-895. CrossRef
McCann, M.C., Wells, B., Roberts, K., Direct visualization of cross-links in the primary plant cell wall, J. Cell Sci. 96 (1990) 323-334.
Konishi, Y., Kitazato, S., Asano, R., Nakatani, N., Polymorphism of acid and neutral-glucosidases in banana pulp: changes in apparent pIs and affinity to Con A of enzymes during ripening, Agr. Biol. Chem. Tokio 55 (4) (1991) 1089-1094.
Markovic, O., Heinrichova, H., Lenkey, B., Pectilytic enzymes from banana, Collect. Czech. Chem. Commun. 40 (1975) 769-774. CrossRef
Biggs, A.R., El-Kholi, M.M., El-Neshawy, S., Nickerson, R., Effects of calcium salts on growth, polygalacturonase activity and infection of peach fruit by Monilinia fructicola, Plant Dis. 81 (4) (1997) 399-403. CrossRef
Siddiqui, S., Bangerth, F., Differential effect of calcium and strontium on flesh firmness and properties of cell walls in apples, J. Hortic. Sci. 70 (6) (1995) 949-953. CrossRef
Cooper, R.M., Rankin, B., Wood, R.K.S., Cell wall degrading enzymes of vascular wilt fungi. 11. Properties and modes of action of polysaccharidases of Verticillium alboatrum and Fusarium oxysporum f. sp. lycopersici, Physiol. Plant Pathol. 13 (1978) 101-134. CrossRef
Guevara, M.A., Gonzalez-Jaen, M.T., Estevez, P., Multiple forms of pectic lyases and polygalacturonase from Fusarium oxysporum: f. sp. radicis lycopersici: regulation of their synthesis by galacturonic acid, Can. J. Microbiol. 43 (3) (1997) 245-253. CrossRef
Medina-Suarez, R., Manning, K., Fletcher, J., Aked, J., Bird, C.R., Seymour, G.B., Gene expression in the pulp of ripening bananas, Plant Physiol. 115 (1997) 453-461. CrossRef