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Polyphenol oxidase activity in mango (Mangifera indica L. )in relation to flowering behaviour and the malformation incidence

Published online by Cambridge University Press:  15 April 2002

Ram Roshan Sharma ,
Chhavi Nath Singh  and
Apurba Millan Goswami
Ram Roshan Sharma
Affiliation:
Division of Fruits and Horticultural Technology, Indian Agricultural Research Institute, New Delhi-110012, India
Chhavi Nath Singh
Affiliation:
Department of Horticulture, CCR College, Muzaffarnagar, U.P., India
Apurba Millan Goswami
Affiliation:
Division of Fruits and Horticultural Technology, Indian Agricultural Research Institute, New Delhi-110012, India
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Abstract

Introduction. Biennial bearing and malformation are the two present problems of mango which are threatening the very existence of a global mango industry in India. Many factors are closely associated with these problems but, presently, those really responsible are not known exactly. Polyphenol oxidase (PPO) plays vital roles in higher plants and, hence, its activity was measured in swollen buds at panicle initiation in regular and biennial bearing mango cultivars to establish a relation between the PPO activity and the flowering behaviour or the malformation incidence. Materials and methods. Six regular (Sensation, Eldon, Tommy Atkins, Amrapali, Neelum and Bangalora) and six biennial bearing cultivars (Langra, Chausa, Edward, St. Alexandrina, Extrema and Bombay Green) were studied. PPO activity was measured in fully swollen buds by preparing a crude enzyme extract at 4 °C. Catecholase activity was measured by using 4-methyl catechol (4-MC) as the substrate, while 4-methyl phenol (p-cresol) was used for cresolase. Results and discussion. Both catecholase and cresolase activities were higher in the swollen buds in the year 2000 than in 1999. Regular bearers exhibited low PPO activity and showed the higher incidence of malformation as compared to the biennial bearing cultivars.Whatever their type of production, cultivars exhibited significant variations in PPO activities and malformation incidence. Conclusion. A strong negative correlation between PPO activity and number of panicles, and PPO activity and malformation incidence, suggests that the PPO activity is inversely related to both flowering and malformation incidence in mango.

Keywords

IndiaMangifera indicaproductionenzymic activitycatechol oxidasefloweringmalformationsfruitsIndeMangifera indicaproductionfréquence de récolteactivité enzymatiquecatéchol oxydasefloraisonmalformationfruits
Type
Research Article
Information
Fruits , Volume 56 , Issue 4 , July 2001 , pp. 219 - 224
Copyright
© CIRAD, EDP Sciences

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References

Pandey S.N., Mango cultivars, In: Srivastava R.P. (Ed), Mango cultivation, International Book Distributing Company, Lucknow, India, 1998.
Singh, L.B., Biennial bearing in mango as affected by cultural operations, weather conditions and the tree vigour, Hortic. Adv. 5 (1960) 96-100.
Naik, K.C., Rao, M.M., Some factors governing fruit bud formation in mango (Mangifera indica L.). II. Relation between growth and fruiting, Madras Agr. J. 30 (1942) 365-370.
Roy, R.S., Studies on irregular bearing of mango (Mangifera indica L.), Indian J. Hortic. 10 (1953) 157-160.
Sen, P.K., Sen, S.K., Guha, D., Carbohydrate and nitrogen content of mango shoots in relation to fruit bud differentiation in them, Indian Agr. 7 (1963) 133-138.
Chacko E.K., Studies on physiology of flowering and fruit growth in mango (Mangifera indica L.), IARI, Thesis, New Delhi, India, 1968.
Singh, R.N., Majumder, P.K., Sharma, D.K., Rathore, D.S., Effect of defruiting on bearing behaviour of mango (Mangifera indica L.) cv. Dashehari, Indian J. Hortic. 25 (1968) 64-65.
Majumder P.K., Sharma D.K., Mango, In: Bose T.K., Mitra S.K. (Eds), Fruits: tropical and sub-tropical fruits, Naya Prokash Publishers, Calcutta, India, 1985.
Rao, M.M., Srihari, D., Approaches for managing the biennial bearing in Alphonso mango trees, J. Maharashtra Agr. Univ. 23 (1998) 19-21.
Mayer, A.M., Harel, E., Polyphenol oxidase in higher plants: a review, Phytochemistry 18 (1979) 193-215. CrossRef
Gordon, S.A., Paleg, L.G., Formation of auxin from tryptophan through the action of polyphenols, Plant Physiol. 36 (1961) 836-845. CrossRef
Briggs, W.R., Ray, P.M., An auxin inactivation system involving tyrosinase, Plant Physiol. 51 (1976) 165-167.
Tomaszewski, M., Thimman, K.V., Interactions of phenolic acids, metallic ions and chelating agents on auxin induced growth, Plant Physiol. 41 (1966) 1443-1454. CrossRef
Pandey R.M., Rao M.M., Pathak R.K., Biochemical changes associated with floral malformation, Scientia Hortic. 11 ( 1977) 37-44.
Ram S., Yadav V.K., Mango malformation. A review, J. Appl. Hortic. 1(1999) 70-78.
Sharma R.R., Goswami A.M., Singh C.N., Chhonkar O.P., Singh, G., Catecholase and cresolase activities and phenolic content in mango (Mangifera indica L.) at panicle initiation, Scientia Hortic. 87 (1/2) (2001) 147-151.
Panse V.G., Sukhatme P.V., Statistical methods for agricultural workers, 3 $^{{\rm rd}}$ ed., Indian Council of Agricultural Research (ICAR) Publication, New Delhi, India, 1978.
Vaughan, K.C., Duke, S.O., Function of polyphenol oxidase in higher plants, Physiol. Plantarum 60 (1984) 106-12. CrossRef
Valero, E., Sanchez-Ferrer, A., Varon, V., Garcia-Carmona, F., Evolution of grape polyphenol oxidase activity and phenolic content pattern during maturation and vinification, Vitis 28 (1989) 85-95.