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Organization of morphological and genetic diversity of Caribbean and Venezuelan papaya germplasm

Published online by Cambridge University Press:  15 April 2006

John Ocampo ,
Géo Coppens d’Eeckenbrugge ,
Saturnin Bruyère ,
Luc de Lapeyre de Bellaire  and
Patrick Ollitrault
John Ocampo
Affiliation:
Cirad, Station de Neufchâteau, Sainte-Marie, 97130 Capesterre-Belle-Eau, Guadeloupe, France
Géo Coppens d’Eeckenbrugge
Affiliation:
Cirad, Boulevard de la Lironde, TS 50 / PS4, 34398 Montpellier Cedex 5, France
Saturnin Bruyère
Affiliation:
Cirad, Station de Neufchâteau, Sainte-Marie, 97130 Capesterre-Belle-Eau, Guadeloupe, France
Luc de Lapeyre de Bellaire
Affiliation:
Cirad, Station de Neufchâteau, Sainte-Marie, 97130 Capesterre-Belle-Eau, Guadeloupe, France
Patrick Ollitrault
Affiliation:
Cirad, Boulevard de la Lironde, TS 50 / PS4, 34398 Montpellier Cedex 5, France
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Abstract

Introduction. The severe impact of papaya bacterial canker in the West Indies justified launching a program to transfer the genetic resistance/tolerance identified in local germplasm to good-quality cultivars. As a first step, a germplasm collection from the Caribbean was gathered and its morphological and genetic diversity assessed. Materials and methods. The total sample included 50 accessions from the Lesser Antilles, Cuba, Venezuela, Costa Rica and Hawaii. Morphological data from a subsample of 29 accessions were submitted to analysis of variance, principal component analysis and Neighbor-Joining cluster analysis. Nine isozyme systems were tested, and the corresponding data were processed for cluster analysis and genetic parameters, including the fixation index, diversity indices and heterozygosity. Results. Eighteen of the 47 Caribbean and Venezuelan accessions exhibited hermaphroditism. Venezuelan accessions displayed the widest morphological diversity, while those from Barbados were distinguished by fruit quality. Sexual type affected leaf and flower traits. Cluster analysis shows little morphological differentiation between origins, with the relative grouping of the accessions from Guadeloupe or Barbados. Only four isozyme systems were polymorphic, producing 34 distinct zymotypes, with a relatively high heterozygosity and a particular diversity in Venezuela, Guadeloupe and Barbados. Geographic structuration appears limited in the cluster analysis among individuals; however, it is clearer when considering the six best-represented populations, with one cluster representing Venezuela, Trinidad and Barbados, and one for Guadeloupe, Martinique and Grenada. Discussion. The low level of fixation is attributed to dioecy and to the low level of selection. The relative isolation of island populations has favored geographic differentiation, albeit limited. The differentiation between the good-quality germplasm from Barbados and the tolerant populations from Martinique and Guadeloupe encourages the development of a marker-assisted introgression scheme.

Keywords

CaribbeanVenezuelaCarica papayagermplasmisoenzymesCaraïbesVénézuélaCarica papayagermplasmisoenzyme
Type
Research Article
Information
Fruits , Volume 61 , Issue 1 , January 2006 , pp. 25 - 37
Copyright
© CIRAD, EDP Sciences, 2006

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References

Webb, R.R., Epidemiology and control of bacterial canker of papaya caused by an Erwinia sp. on St. Croix US Virgin Islands, Plant Dis. 69 (4) (1985) 305–309.
Gardan, L., Christen, R., Achouak, W., Prior, P., Erwinia papayae sp. Nov., a pathogen of papaya (Carica papaya), Int. J. Syst. Evol. Microbiol. 54 (2004) 107–113. CrossRef
Frossard, P., Un dépérissement du papayer aux Antilles françaises associé à une Erwinia sp. du groupe amylovora, Fruits 40 (1985) 583–595.
Prior, P., Béramis, M., Rousseau, M.T., Le dépérissement bactérien du papayer aux Antilles françaises, Agronomie 5 (10) (1985) 877–885. CrossRef
De Lapeyre de Bellaire, L., Lyannaz, J.P., Evidence for resistance sources to bacteria canker in local Guadeloupe populations of Carica papaya, Trop. Fruit. Newsl. 3 (1992) 6–7.
Manshardt, R.M., Zee, F.T.P., Papaya germplasm and breeding in Hawaii, Fruit Var. J. 48 (3) (1994) 146–152.
Alvarez R., Gómez G., Martínez E., Mejoramiento genético de la papaya Carica papaya L. por ciclos de selección y recombinación en poblaciones avanzadas de híbridos varietales, in: V. Congreso Sociedad Colombiana de Fitomejoramiento y Producción de Cultivos: Memorias La sostenibilidad y la competitividad en el desarrollo de los cultivos, Santa Marta, Colombia, 1997, p 29.
Sánchez E., Samaniego J.A., Comportamiento fenológico de variedades de papayo en el sur de Sonora, in: XVII Congreso de Fitogenética, Acapulco, Mexico, 1998.
Somsri S., Improvement of papaya (Carica papaya L.) for South-East Queensland: investigation of sex-type and fruit quality, Aust. New Crop. Newsl. 11 (25.2) (1999).
Nakasone H.Y., Storey W.B., Studies on the inheritance of fruiting height of Carica papaya L., Am. Soc. Hortic. Sci. Proc., 66, 1955, pp. 168–182.
Tan, S.C., Weinheimer, E.A., The isoenzyme patterns of developing fruit and leaf of papaya (Carica papaya L.), Sains Malays. 5 (1976) 7–14.
Morshidi, M., Genetic control of isozymes in Carica papaya L., Euphytica 103 (1) (1998) 89–94. CrossRef
Morshidi, M., Manshardt, R.M., Zee, F., Isozyme variability in wild and cultivated Carica papaya, HortScience 30 (1995) 809.
Jobin-Decor, M. P., Graham, G.C., Henry, R.J., Drew, R.A., RAPD and isozyme analysis of genetic relationships between Carica papaya and wild relatives, Gen. Res. Crop Evol. 44 (5) (1997) 471–477. CrossRef
Manshardt, R.M., Wenslaff, T.F., Zygotic polyembryony in interspecific hybrids of Carica papaya and C. cauliflora, J. Am. Soc. Hortic. Sci. 114 (4) (1989) 684–689.
Moore, G.A., Litz, R.E., Biochemical markers for Carica papaya, C. cauliflora, and plants from somatic embryos of their hybrid, J. Am. Soc. Hortic. Sci. 109 (2) (1984) 213–218.
Manshardt, R.M., Wenslaff, T.F., Interspecific hybridation of papaya with other Carica species, J. Am. Soc. Hortic. Sci. 114 (4) (1989) 689–694.
Chen, M.H., Wang, C.C., Wang, D.N., Chen, F.C., Somatic embryogenesis and plant regeneration from immature embryos of Carica papaya × C. cauliflora L. cultured in vitro, Can. J. Bot. 69 (9) (1991) 1913–1918. CrossRef
Anon., Descriptors-for-papaya, IBPGR, FAO, Rome, Italy, 1988.
Saitou, N., Nei, M., The neighbor-joining method: a new method for reconstructing phylogenetic trees, Mol. Biol. Evol. 4 (1987) 406–425.
Wright, S., The interpretation of population structure by F-statistics with special regard to system of mating, Evolution 13 (1965) 395–420. CrossRef
Nei, M., Analysis of gene diversity in subdivided population, Proc. Natl. Acad. Sci. US 70 (1973) 3321–3323. CrossRef
Sondur, N.S., Manshardt, R.M., Stliles, J.I., A genetic linkage map of papaya based on randomly amplified polymorphic DNA markers, Theor. Appl. Genet. 93 (4) (1996) 547–553. CrossRef
Stiles, J.I., Lemme, C., Sondur, S., Morshidi, M.B., Manshardt, R.M., Using randomly amplified polymorphic DNA for evaluating genetic relationships among papaya cultivars, Theor. Appl. Genet. vol. 85 (6–7) (1993) 697–701.
Kim, M.S., Moore, P.H., Zee, F., Fitch, M.M.M., Steiger, D.L., Manshardt, R.M., Paull, R.E., Drew, R.A., Sekioka, T., Ming, R., Genetic diversity of Carica papaya as revealed by AFLP markers, Genome 45 (3) (2002) 503–512. CrossRef