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Nursery hardening of in vitro-produced banana plants

Published online by Cambridge University Press:  08 December 2009

John Charles Robinson  and
Victor Galán Sáuco
John Charles Robinson
Affiliation:
Du Roi Lab., P.O. Box 1147, Letsitele 0885, South Africa
Victor Galán Sáuco
Affiliation:
Inst. Canario Investig. Agrar. (ICIA), Apartado Correos 60, 38208 La Laguna, Tenerife, Canary Islands, Spain
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Abstract

Introduction. This protocol can be used as a guide for people who wish to construct their own nursery. It describes the methods for receiving, transplanting, managing and hardening young banana tissue culture plants after the weaning stage. The principle, key advantages, starting plant material and time required are presented. Materials and methods. This part describes the nursery construction, the nursery media, fertiliser and bags, the handling and transplanting of weaned plants into bags (3 steps), and the management schedule for hardening nursery plants (8 steps). Possible troubleshooting is listed. Results. All the characteristics of nursery-hardened plants in bags, ready for transplanting to the field, are formulated.

Keywords

South AfricaSpainMusa sp.methodsvitroplantshardeningAfrique du SudEspagneMusa sp.méthodevitroplantdurcissement
Type
Research Article
Information
Fruits , Volume 64 , Issue 6 , November 2009 , pp. 383 - 392
Copyright
© CIRAD, EDP Sciences, 2009

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References

Robinson, J.C., Galán Sauco, V., Weaning (acclimatization) of in vitro-produced banana plants, Fruits 64 (2009) 325–332. CrossRef
Israeli Y., Lahav E., Reuveni O., In-vitro culture of bananas, in: Gowen S. (Ed.), Bananas and Plantains, Chapman and Hall, Lond., UK, 1995, pp. 147–178.
Arias, O., Valverde, M., Producción y variación somaclonal de plantas de banano variedad Grande Naine producidas por cultivo de tejidos, Asbana (San Jose, Costa Rica) 28 (1987) 6–11.
Hwang, S.C., Chen, C.L., Lin, J.C., Lin, H.L., Cultivation of banana using plantlets from meristem culture, HortSci. 19 (1984) 231–233.
Vuylsteke D.R., Talengera D., Post-flask management of micropropagated bananas and plantains, IITA, Ibadan, Nigeria, 1998, 15 p.
Vuylsteke D.R., Shoot tip culture for the propagation, conservation and distribution of Musa germplasm, IITA, Ibadan, Nigeria, 1998, 82 p.
Israeli Y., Reuveni O., Nameri N., Genetic variability and performance of in-vitro propagated banana plants, in: Chaves J.A., Calderon R.R. (Eds.), Memorias 1986 de la IV Reunion sobre Agrofisiologia del Banano, Asbana, San Jose, Costa Rica, 1988, pp. 97–104.
Daniells J.W., Smith M.K., Post-flask management of tissue-cultured bananas, Tech. Rep. 18, ACIAR, Canberra, Aust., 1991, 9 p.
Daniells J.W., Smith M.K., Hamill S.D., banana offtypes, an illustrated guide, Inf. Ser. Q199019, Dep. Prim. Ind., Qld., Aust., 1999, 10 p.
Côte F.X., Sandoval J., Marie P., Auboiron E., Variations in micropropagated bananas and plantains: literature survey, Fruits 48 (1993)15–23.
Robinson, J.C., General recommendations for managing tissue culture banana plants in the field, Inf. Bull. (Inst. Trop. Subtrop. Crops, Nelspruit) 257 (1994) 1–5.