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Mesure de l’activité antiradicalaire du jus et des peaux d’oranges tunisiennes par le radical DPPH

Published online by Cambridge University Press:  03 June 2006

Jamilla Kalthoum Cherif ,
Ines M’Rabet ,
Mourad El Habiri ,
Rym Abidi  and
Anne-Marie Albrecht-Gary
Jamilla Kalthoum Cherif
Affiliation:
Institut préparatoire aux études d'ingénieurs de Tunis, 1008 Monfleury, Tunis, Tunisie
Ines M’Rabet
Affiliation:
Faculté des sciences de Bizerte, Université du 7 novembre à Carthage, 7021 Jarzouna–Bizerte, Tunisie École Chimie Polymère Matériaux, Laboratoire de physico-chimie bioinorganique, UMR 7509 CNRS, 25, rue Becquerel, 67200 Strasbourg, France
Mourad El Habiri
Affiliation:
Faculté des sciences de Bizerte, Université du 7 novembre à Carthage, 7021 Jarzouna–Bizerte, Tunisie
Rym Abidi
Affiliation:
École Chimie Polymère Matériaux, Laboratoire de physico-chimie bioinorganique, UMR 7509 CNRS, 25, rue Becquerel, 67200 Strasbourg, France
Anne-Marie Albrecht-Gary
Affiliation:
Faculté des sciences de Bizerte, Université du 7 novembre à Carthage, 7021 Jarzouna–Bizerte, Tunisie
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Abstract

Introduction. L’orange (Citrus aurantium sinensis) est une source de composés bioactifs : vitamines C et E, flavonoïdes (flavanes et flavanones glycosylés et polyméthoxylés). Pour valoriser les déchets des peaux d’orange, nous avons mesuré l’activité antiradicalaire du jus et des peaux sèches et fraîches de cinq variétés d’orangers de Tunisie afin d’évaluer leur concentration en composés antioxydants utilisables dans l’industrie. Matériel et méthodes. Le pouvoir antiradicalaire du jus et des extraits des peaux a été évalué en suivant, en fonction du temps, la disparition du radical stable DPPH• par spectrophotométrie d’absorption UV-visible à 515 nm. L’activité antiradicalaire mesurée a été exprimée en concentration efficace (CE50). La détermination du volume V50 de vitamine C d’une solution d’acide ascorbique de concentration connue et d’un volume V50 des extraits de jus et des peaux sèches et fraîches d’orange a permis d’estimer la concentration molaire et massique de vitamine C équivalente dans les extraits. Résultats. Les jus d’orange contiennent environ 400 mg·L–1 d’équivalent de vitamine C. Dans le cas de réactions très rapides avec le radical DPPH•, la totalité de l’activité antiradicalaire peut être attribuée à la vitamine C. Dans le cas de réactions plus lentes, en plus de la vitamine C, ce sont les polyphénols (citroflavonoïdes) qui contribuent à l’activité antioxydante totale. Les extraits méthanoliques des peaux fraîches (flavédo) contiennent plus de vitamine C que les extraits de peaux sèches. Conclusion. Les peaux d’oranges constituent d’immenses sources d’antioxydants qui peuvent trouver leur application dans différents domaines : agroalimentaire, cosmétique, pharmaceutique et autres.

Keywords

TunisiaCitrus sinensisfruitsorange juicepeelwaste utilizationnatural antioxidantsascorbic acidTunisieCitrus sinensisfruitsjus d'orangepelureutilisation des déchetsantioxydant naturelacide ascorbique
Type
Research Article
Information
Fruits , Volume 61 , Issue 2 , March 2006 , pp. 99 - 107
Copyright
© CIRAD, EDP Sciences, 2006

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