In vitro rearing of Pratylenchidae nematodes on carrot discs

Abstract

Introduction. This rearing technique is applicable to migratory nematodes (e.g., Pratylenchus spp. and Radopholus similis) for mass production of nematodes for experimental purposes, conservation of nematodes, and direct studies of nematodes’ multiplication or reproduction mechanisms. The principle of the method applied, key advantages, starting plant material and time required are presented. Materials and methods. Necessary laboratory materials, and details of the nine steps required for the preparation of carrot discs, preparation of nematodes, inoculation of nematodes and collection of nematodes out of petri dishes are described. Possible troubleshooting is explained. Results. Several thousands of nematodes can be extracted out of one carrot disc after 6–8 weeks of culture, depending on nematode species and geographical population.