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Improving pineapple micropropagation protocol through explant size and medium composition manipulation

Published online by Cambridge University Press:  15 April 2002

Lirio Luiz Dal Vesco ,
Adelar de Almeida Pinto ,
Gilmar Roberto Zaffari ,
Rubens Onofre Nodari ,
Maurício Sedrez dos Reis  and
Miguel Pedro Guerra
Lirio Luiz Dal Vesco
Affiliation:
Universidade Federal de Santa Catarina, CCA, Departamento de Fitotecnia, Florianópolis, SC, Cx. Postal 476 - CEP 88034-001, Brazil
Adelar de Almeida Pinto
Affiliation:
Universidade Federal de Santa Catarina, CCA, Departamento de Fitotecnia, Florianópolis, SC, Cx. Postal 476 - CEP 88034-001, Brazil
Gilmar Roberto Zaffari
Affiliation:
Estação Experimental de ItajaÌ (EPAGRI), C.P. 277, Itajaí, SC. 88.301-970, Brazil
Rubens Onofre Nodari
Affiliation:
Universidade Federal de Santa Catarina, CCA, Departamento de Fitotecnia, Florianópolis, SC, Cx. Postal 476 - CEP 88034-001, Brazil
Maurício Sedrez dos Reis
Affiliation:
Universidade Federal de Santa Catarina, CCA, Departamento de Fitotecnia, Florianópolis, SC, Cx. Postal 476 - CEP 88034-001, Brazil
Miguel Pedro Guerra
Affiliation:
Universidade Federal de Santa Catarina, CCA, Departamento de Fitotecnia, Florianópolis, SC, Cx. Postal 476 - CEP 88034-001, Brazil
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Abstract

Introduction. Although several pineapple micropropagation protocols have already been published, significant improvement could be achieved if the stages of the in vitro culture were better defined. Our work concerned several experiments aiming at the mass production of high quality plantlets. Materials and methods. Axillary buds were inoculated on an MS liquid culture medium added with NAA (2 μM) and BAP (4 μM). Regenerated shoots, divided into three classes of different sizes, were then used in further experiments. First, these shoots were inoculated in flasks containing the same MS culture medium with or without growth regulators. Then, four basic media, containing different salts and free from growth regulators were tested. In a third assay, the MS culture medium was compared with a half-diluted MS culture medium for studying the plantlet elongation and rooting stage. Results. In the MS culture medium supplemented with NAA and BAP, the highest multiplication rate (13.5 shoots) was obtained with the smallest shoots inoculated, while in the MS culture medium free of growth regulators, the highest plantlets (7.7 cm) were the result of the highest shoots inoculated and showed no vitrification. The normal MS culture medium, in comparison with the half-diluted one and the three other salt formulations, revealed a significant increase in the plantlet elongation and best general features. For acclimatization, the highest values of the survival rate (93.8% ) and fresh and dry weights were obtained with the transference of higher than 7.0 cm in vitro plantlets. Conclusion. Using the protocol described in this work, it is possible to obtain 1 million in vitro plantlets after 9 months from a single bud, with a 45 day subculture interval and an average multiplication rate of 10 shoots per bud.

Keywords

BrazilAnanas comosusplant propagationmicropropagationin vitro experimentationculture mediatransplantingplant establishmentBrésilAnanas comosusmultiplication des plantesmicropropagationexpérimentation in vitromilieu de culturerepiquageétablissement de la plante
Type
Research Article
Information
Fruits , Volume 56 , Issue 3 , May 2001 , pp. 143 - 154
Copyright
© CIRAD, EDP Sciences

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References

Cabral, J.R.S., Matos, A.P., Cunha, G.A.P., Selection of pineapple cultivars resistant to fusariose, Acta Hortic. 334 (1993) 53-58. CrossRef
Cabral, J.R.S., Matos, A.P., Pineapple breeding for resistance to fusariosis in Brazil, Rev. Fac. Agron. Maracay 21 (1995) 137-145.
Leal F, Coppens d'Eckenbrugge G., Pineapple, in: Janick J., Moore J.N. (Eds.), Fruit Breeding - Tree and Tropical Fruits, John Wiley, New York, 1996, pp. 515-557.
Aghion, D., Beauchesne, G., Utilisation de la technique de culture stérile d'organes pour obtenir des clones d'ananas, Fruits 15 (1960) 464-466.
Rangan T.S., Pineapple, In: Ammirato P.V., Evans D.A., Sharp W.R., Yamada Y. (Eds), Handbook of plant cell culture, Macmillan Publishing Co, New York, 1984, pp. 373-82.
Dewald, M.G., Moore, G.A., Sherman, W.B., Evans, M.H., Production of pineapple in vitro, Plant Cell Rep. 7 (1988) 535-537. CrossRef
Fitchet, M., Clonal propagation of Queen and Smooth Cayenne pineapples, Acta Hortic. 275 (1990) 261-266. CrossRef
Hirimburegama, K., Wijesinghe, L.P.J., In vitro growth of Ananas comosus L. Merr. (pineapple) shoot apices on different media, Acta Hortic. 319 (1992) 203-208. CrossRef
Fitchet-Purnell, M., Maximum utilization of pineapple crowns for micropropagation, Acta Hortic. 334 (1993) 325-330. CrossRef
Kiss, E., Kiss, J., Gyulai, G., Heszky, L.E., A novel method for rapid micropropagation of pineapple, HortSciences 30 (1) (1995) 127-129.
Daquinta, M., Benega, R., Brief review of tissue culture in pineapple, Pineapple News 3 (1) (1997) 7-9.
Almeida, W.A.B. de, Matos, A.P. de, Souza, A. da S., Effects of benzylaminopurine (BAP) on in vitro proliferation of pineapple (Ananas comosus (L.) Merr.), Acta Hortic. 425 (1997) 235-242. CrossRef
Teng, W.L., An alternative propagation method of Ananas through nodule culture, Plant Cell Rep. 16 (1997) 454-457.
Guerra, M.P., Dal Vesco, L.L., Pescador, R., Schuelter, A.R., Nodari, R.O., Establishment of a regenerative protocol for the pineapple micropropagation, Pesq. Agropecu. Bras. 34 (9) (1999) 1557-1563. CrossRef
Wakasa K., Pineapple (Ananas comosus L. Merr.), in: Bajaj Y.P.S. (Ed), Biotechnology in Agriculture and Forestry - Trees II, Springer-Verlag, Berlin, Germany, 1989, pp.13-29.
Fitchet, M., Organogenesis in callus cultures of pineapples (Ananas comosus (L.) Merr., Acta Hortic. 275 (1990) 267-274. CrossRef
Benega, R., Isidrón, M., Arias, E., Cisneros, A., Companioni, L., Martínez, J., Borroto, C.G., Plant regeneration from pineapple (Ananas comosus L. Merr.) ovules, Acta Hortic. 425 (1997) 247-250. CrossRef
Mayak, S., Tirosh, T., Ilan, A., Duvdevani, A., Khayat, E., Growth and development of pineapple (Ananas comosus L.) plantlets cultured in vitro at enriched and ambient CO2 environments, Acta Hortic. 461 (1998) 225-229. CrossRef
Debergh P.C., Read P.E., Micropropagation, in: Debergh P.C., Zimmerman R.H. (Eds.), Micropropagation - Technology and application, Kluwer Academic Pu., Dordrecht, Netherlands, 1991, pp. 1-13.
George E.F., Plant propagation by tissue culture, Exegetics, Edington, UK, Part 1, 1993, 574 p.
Krikorian A.D., Hormones in tissue culture and micropropagation, in: Davies P.J. (Ed.), Plant hormones, Kluwer Academic Pu, Dordrecht, Netherlands,1995, pp. 774-796.
Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plantarum 15 (1962) 473-497. CrossRef
von Arnold, S., Eriksson, T., In vitro studies of adventitious shoot formation in Pinus contorta, Can. J. Bot. 59 (1981) 870-874. CrossRef
Chu C.C., The N6 medium and its applications to anther culture of cereal crops, in: Proceedings symposium on plant tissue culture, Pitman Advanced Pu. Program, Boston, USA, 1981, pp. 43-50.
Knudson, L., A new nutrient solution for germination of orchid seed, Am. Orchid Soc. Bull. 14 (1946) 214-217.
Steel R.G.D., Torrie J.H., Principles and procedures of statistics - A biometrical approach, Mcgraw-Hill Book Co, New York, USA, 1980, 633 p.
Compton, M., Statistical methods suitable for the analysis of plant tissue culture data, Plant Cell Tiss. Org. 37 (1994) 217-242.
Gaspar, T., The concept of cancer in in vitro plant cultures and the implication of habituation to hormones and hyperhydricity, Plant Tiss. Cult. Biotechnol. 1 (3) (1995) 126-136.
Marga, F., Vebret, L., Morvan, H., Agar fractions could protect apple shoots cultured in liquid media against hyperhydricity, Plant Cell Tiss. Org. 49 (1997) 1-5. CrossRef
Nobre, J., In vitro cloning and micropropagation of Lavandula stoechas from field-grown plants, Plant Cell Tiss. Org. 46 (1996) 151-155. CrossRef
Ziv M., Vitrification, morphological and physiological disorders of in vitro plants, in: Debergh P.C., Zimmerman R.H. (Eds), Micropropagation - Technology and application, Kluwer Academic Pu, Dordrecht, Netherlands, 1991, pp. 45-69.
Larkin, P.J., Scowcroft, W.R., Somaclonal variation - A novel source of variability from cell cultures for plant improvement, Theor. Appl. Genet. 60 (1981) 197-214. CrossRef
Peschke, V.M., Phillips, R.L., Genetic implications of somaclonal variation in plants, Adv. Genet. 30 (1992) 41-75.
Zhu, J., Bartholomew, D.P., Goldstein, G., Effect of elevated carbon dioxide on the growth and physiological responses of pineapple, a species with crassulacean acid metabolism, J. Am. Soc. Hortic. Sci. 122 (2) (1997) 233-237.
Vasil, I.K., Automation of plant propagation, Plant Cell Tiss. Org. 39 (1994) 105-108. CrossRef
George E.F., Plant propagation by tissue culture, Exegetics, Edington, UK, Part 2, 1996, 699 p.
Preece, J.E., Can nutrient salts partially substitute for plant growth regulators?, Plant Tiss. Cult. Biotechnol. 1 (1) (1995) 26-36.
Murashige T., Tucker D.P.H., Growth factor requirements of Citrus tissue culture, in: Chapman H.D. (Ed.), Proc. 1st Int. Citrus Symp. V.3., Univ. Calif. Riverside, Riverside, Ca, USA, 1969, pp. 1115-1161.
Preece J.E., Sutter E.G., Acclimatization of micropropagated plants to the greenhouse and field, in: Debergh P.C., Zimmerman R.H. (Eds), Micropropagation - Technology and application, Kluwer Academic Pu, Dordrecht, Netherlands, 1991, pp. 71-93.
Van Huylenbroeck, J.M., Debergh, P.C., Physiological aspects in acclimatization of micropropagated plantlets, Plant Tiss. Cult. Biotechnol. 2 (3) (1996) 136-141.
Thorpe T.A., Harry I.S., Application of tissue culture to horticulture, Acta Hortic. 447 (1997), 39-49. CrossRef