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Artificial inoculation on plants and banana leaf pieces with Mycosphaerella spp., responsible for Sigatoka leaf spot diseases

Published online by Cambridge University Press:  22 October 2008

Catherine Abadie ,
Marie-Françoise Zapater ,
Luc Pignolet ,
Jean Carlier  and
Xavier Mourichon
Catherine Abadie
Affiliation:
CIRAD, UPR Mult. Vég., Stn. Neufchâteau, 97130, Capesterre Belle-Eau, Guadeloupe, France
Marie-Françoise Zapater
Affiliation:
CIRAD, UMR BGPI, TA A 54/K, 34398, Montpellier Cedex 5, France
Luc Pignolet
Affiliation:
CIRAD, UMR BGPI, TA A 54/K, 34398, Montpellier Cedex 5, France
Jean Carlier
Affiliation:
CIRAD, UMR BGPI, TA A 54/K, 34398, Montpellier Cedex 5, France
Xavier Mourichon
Affiliation:
CIRAD, UMR BGPI, TA A 54/K, 34398, Montpellier Cedex 5, France
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Abstract

Introduction. This protocol aims at (i) analysing the nature of host-parasite interactions (resistance or susceptibility), and (ii) studying the variability of the pathogenicity of different pathogen populations (intra/inter-specific) with regard to both virulence and aggressiveness using artificial inoculation with Mycosphaerella fijiensis or Mycosphaerella musicola on plants and banana leaf pieces. The principle, key advantages, starting plant material and time required for the method are presented. Materials and methods. Laboratory materials required and details of the sixteen steps of the protocol (conidial inoculum preparation, artificial inoculation on banana plants and artificial inoculation on banana leaf pieces) are described. Possible troubleshooting is discussed. Results. Typical symptoms of the different diseases are obtained after artificial inoculation.

Keywords

FranceMusa sp.Mycosphaerella fijiensisMycosphaerella musicoladisease resistancemethodsFranceMusa sp.Mycosphaerella fijiensisMycosphaerella musicolarésistance aux maladiesméthode
Type
Research Article
Information
Fruits , Volume 63 , Issue 5 , September 2008 , pp. 319 - 323
Copyright
© CIRAD, EDP Sciences, 2008

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References

Fouré É, Mourichon X., Jones D.R., Black leaf streak disease. Evaluating germplasm for reaction to black leaf streak, in: Jones D.R. (Ed.), Diseases of banana, abaca and enset, CABI Publ., Wallingford, UK, 2000, pp. 62–67.
Mourichon, X., Peter, D., Zapater, M.F., Inoculation expérimentale de Mycosphaerella fijiensis Morelet sur jeunes plantules de bananiers issues de cultures in vitro, Fruits 42 (4) (1987) 195–198.
Abadie C., Pignolet L., Elhadrami A., Habas R., Zapater M.F., Carlier C., Inoculation avec Mycosphaerella sp., agent de cercosporioses, de fragments de feuilles de bananiers maintenus en survie, Cah. Tech. INRA ; Numéro spéc. Méthodes d’appréciation du comportement variétal vis-à-vis des bioagresseurs, INRA, Paris, France, 2005, pp. 131–134.