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ENHANCING SEED GERMINATION OF FOUR CROP SPECIES USING AN ULTRASONIC TECHNIQUE

Published online by Cambridge University Press:  08 March 2010

S. J. GOUSSOUS ,
N. H. SAMARAH ,
A. M. ALQUDAH  and
M. O. OTHMAN
S. J. GOUSSOUS*
Affiliation:
Department of Plant Production, Faculty of Agriculture, Jordan University of Science & Technology, PO Box 3030, Irbid 22110, Jordan
N. H. SAMARAH
Affiliation:
Department of Plant Production, Faculty of Agriculture, Jordan University of Science & Technology, PO Box 3030, Irbid 22110, Jordan
A. M. ALQUDAH
Affiliation:
Department of Plant Production, Faculty of Agriculture, Jordan University of Science & Technology, PO Box 3030, Irbid 22110, Jordan
M. O. OTHMAN
Affiliation:
Department of Mechanical Engineering, Jordan University of Science & Technology, PO Box 3030, Irbid 22110, Jordan
*
‡Corresponding author. [email protected]
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Summary

A laboratory experiment was conducted to determine the effect of ultrasound (US) treatment on seed germination of chickpea, wheat, pepper and watermelon. All tests were carried out at 40 kHz in a water bath ultrasonic device varying two factors, treatment duration (5, 10, 15, 30, 45 or 60 min) and germination temperature (15 or 20 °C). Parallel tests were run in which seeds were soaked in water without sonication in order to eliminate the effect of water from US test results. The effects of US on seed germination varied between crops and were more obvious on germination speed, expressed as germination rate index (GRI), rather than on germination percentage (GP). In particular, US treatment significantly increased the GRI of chickpeas, wheat and watermelon, resulting in a maximum increase of 133% (at 45 min), 95% (30 min) and 45% (5 min), respectively, above control seeds. The beneficial effects of US on the GRI of these crops were observed at both 15 and 20 °C, suggesting that US treatment offers a practical priming method to overcome the slow germination that may occur at low temperatures. Water-soaking treatment improved the GP of both chickpea and pepper seeds by 59 and 24%, respectively, compared to the control but neither water nor US had any positive effect on pepper GRI. Post-treatment measurement of moisture content of these seeds produced variable results depending on crop species and US treatment duration. Results of this research indicated that US treatment effectively enhanced speed of germination of chickpea, wheat and watermelon seeds. This increase in speed of germination may improve early field establishment of these crops in the semiarid Mediterranean region and thus needs further investigation. The US technique may also be very useful for plant propagators in nurseries to achieve fast seedling establishment of watermelon.

Type
Research Article
Information
Experimental Agriculture , Volume 46 , Issue 2 , April 2010 , pp. 231 - 242
Copyright
Copyright © Cambridge University Press 2010

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References

REFERENCES

Aladjadjiyan, A. (2002). Increasing carrot seeds (Daucus carota L.), cv. Nantes, viability through ultrasound treatment. Bulgarian Journal of Agricultural Science 8: 469–472.Google Scholar
Apar, D. K., Turhan, M. and Özbek, B. (2006). Enzymatic hydrolysis of starch by using a sonifier. Chemical Engineering Communication 193: 1117–1126.CrossRefGoogle Scholar
Ashraf, M. and Foolad, M. R. (2005). Pre-sowing seed treatment: A shotgun approach to improve germination, plant growth and crop yield under saline and non-saline conditions. Advances in Agronomy 88: 223–271.CrossRefGoogle Scholar
AOSA (Association of Official Seed Analysts). (1986). Rules for testing seeds. Journal of Seed Technology 6: 1–125.Google Scholar
Baker, G. K., Robertson, V. J. and Duck, F. A. (2001). A review of therapeutic ultrasound: Biophysical effects. Physical Therapy 81: 1351–1358.CrossRefGoogle ScholarPubMed
Benedito, J., Carcel, J. A., Gonzalez, R. and Mulet, A. (2002). Application of low intensity ultrasonics to cheese manufacturing processes. Ultrasonics 40: 19–23.CrossRefGoogle ScholarPubMed
Burgass, R. W. and Powell, A. A. (1984). Evidence for repair processes in the invigoration of seed by hydration. Annals of Botany 53: 753–757.CrossRefGoogle Scholar
Bort, J., Araus, J. L., Hazzam, H., Grando, S. and Ceccarelli, S. (1998). Relationships between early vigor, grain yield, leaf structure and stable isotope composition in field grown barley. Plant Physiology and Biochemistry 36: 889–897.CrossRefGoogle Scholar
Carbonell, M. V., Martinez, E. and Amaya, J. M. (2000). Stimulation of germination of rice by a static magnetic field. Electro- and Magnetobiology 19: 121–128.CrossRefGoogle Scholar
Demir, I. and Kazim, M. (2004). The effect of priming on seedling emergence of differentially matured watermelon (Citrullus lanatus (Thunb.) Matsum and Nakai) seeds. Scientia Horticulturae 102: 467–473.CrossRefGoogle Scholar
Flórez, M., Carbonell, M. V. and Martinez, E. (2007). Exposure of maize seeds to stationary magnetic fields: Effects of germination and early growth. Environmental and Experimental Botany 59: 68–75.CrossRefGoogle Scholar
Gaba, V., Kathiravan, K., Amutha, S., Singer, S., Xiaodi, X., and Ananthakrishnan, G. (2008). The uses of ultrasound in plant tissue culture. In Focus on Biotechnology, Vol VI. Plant Tissue Culture Engineering, 417–426 (Eds Dutta, G. S. and Ibaraki, Y.). Dordrecht, Netherlands: Springer.Google Scholar
Harris, D., Joshi, A., Khan, P. A., Gothkar, P. and Sodhi, P. S. (1999). On-farm seed priming in semi-arid agriculture: Development and evaluation in maize, rice and chickpea in India using participatory methods. Experimental Agriculture 35: 15–29.CrossRefGoogle Scholar
Harris, D., Raghuwanshi, B. S., Gangwar, J. S., Singh, S. C., Joshi, K. D., Rashid, A. and Hollington, P. A. (2001). Participatory evaluation by farmers of ‘on-farm’ seed priming in wheat in India, Nepal and Pakistan. Experimental Agriculture 37: 403–415.CrossRefGoogle Scholar
Harris, D. (2006). Development and testing of ‘on-farm’ seed priming. Advances in Agronomy 90: 129–178.CrossRefGoogle Scholar
Hebling, S. A. and da Silva, W. R. (1995). Effects of low intensity ultrasound on the germination of corn seeds (Zea mays L.) under different water availabilities. Scientia Agricola 52: 514–520.CrossRefGoogle Scholar
Joersbo, M. and Brunstedt, J. (1992). Sonication: a new method for gene transfer to plants. Physiologia Plantarum 85: 230–234.CrossRefGoogle Scholar
Khan, A. A. (1992). Preplant physiological seed conditioning. Horticultural Reviews 13: 131–181.CrossRefGoogle Scholar
Kaur, S., Gupta, A. K. and Kaur, N. (2005). Seed priming increases crop yield possibly by modulating enzymes of sucrose metabolism in chickpea. Journal of Agronomy and Crop Science 191: 81–87.CrossRefGoogle Scholar
Klein, J. D., Mufradi, I., Cohen, S., Hebbe, Y., Asido, S., Dolgin, B. and Bonfil, D. J. (2002). Establishment of wheat seedlings after early sowing and germination in an arid Mediterranean environment. Agronomy Journal 94: 585–593.CrossRefGoogle Scholar
Korkmaz, A. (2006). Ameliorative effects of ethylene precursor and polyamines on the high temperature inhibition of seed germination in lettuce (Lactuca sativa L.) before and after seed storage, Seed Science and Technology 34: 465–474.CrossRefGoogle Scholar
Maguire, J. D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigour. Crop Science 2: 176–177.CrossRefGoogle Scholar
Mason, T. J. and Povey, M. J. W. (1998). Ultrasound in Food Processing. London: Blackie Academic and Professional.Google Scholar
McDonald, M. B. (2000). Seed priming. In Seed Technology and Its Biological Basis, 287–325 (Eds Black, M. and Bewley, J. D.) Sheffield, UK: Sheffield Academic Press Ltd.Google Scholar
Miyoshi, K. and Mii, M. (1988). Ultrasonic treatment for enhancing seed germination of terrestrial orchid, Calanthe discolor, in asymbiotic culture. Scientia Horticulturae 35: 127–130.CrossRefGoogle Scholar
Moussatov, A., Granger, C. and Dubus, B. (2005). Ultrasonic cavitation in thin liquid layers. Ultrasonics Sonochemistry 12: 415–422.CrossRefGoogle ScholarPubMed
Pill, W. G. (1995). Low water potential and presowing germination treatments to improve seed quality. In Seed Quality, 319–360 (Ed Bastra, A. S.). New York: Food Products Press.Google Scholar
Shimomura, S. (1998). The effects of ultrasonic irradiation on germination. Proceedings Ultrasonic Symposium IEEE 2: 1439–1442.Google Scholar
Shors, J. D., Soll, D. R., Daniels, K. J. and Gibson, D. P. (1999). Method for enhancing germination. US Patent No. 5950362.Google Scholar
Suslick, K. S. (1990). Sonochemistry. Science 247: 1439–1445.CrossRefGoogle ScholarPubMed
Taylor, A. G., Allen, P. S., Bennett, M. A., Bradford, K. J., Burris, J. S. and Misra, M. K. (1998). Seed enhancements. Seed Science Research 8: 245–256.CrossRefGoogle Scholar
Welbaum, G. E. and Bradford, K. J. (1991). Water relations of seed development and germination in muskmelon (Cucumis melo L.). VI. Influence of priming on germination responses to temperature and water potential during development. Journal of Experimental Botany 42: 393–399.CrossRefGoogle Scholar
Yaldagard, M., Mortazavi, S. A. and Tabatabaie, F. (2008a). Influence of ultrasonic stimulation on the germination of barley seed and its alpha-amylase activity. African Journal of Biotechnology 7: 2465–2471.Google Scholar
Yaldagard, M., Mortazavi, S. A. and Tabatabaie, F. (2008b). Application of ultrasonic waves as a priming technique for accelerating and enhancing the germination of barley seed: Optimization of method by the Taguchi approach. Journal of the Institute of Brewing 114: 14–21.CrossRefGoogle Scholar
Zheng, X. Y., Li, X. Q. and Xu, Y. (2007). The effect of hydropriming on germination barriers of triploid watermelon seeds. In Seeds: Biology, Development and Ecology, 269–440 (Eds Adkins, S. C., Navie, S. W., and Ashmore, S.). Wallingford, UK: CABI.Google Scholar